Comparative cytotoxicity of kaolinite, halloysite, multiwalled carbon nanotubes and graphene oxide

doi:10.1016/j.clay.2021.106041

	Comparative cytotoxicity of kaolinite, halloysite, multiwalled carbon nanotubes and graphene oxide
	Rozhina, Elvira 1; Batasheva, Svetlana 1; Miftakhova, Regina 1; Yan, Xuehai 2; Vikulina, Anna 4; Volodkin, Dmitry 3; Fakhrullin, Rawil 1
刊名	APPLIED CLAY SCIENCE
	2021-05-01
卷号	205 页码:10
关键词	Halloysite Kaolinite Multiwalled carbon nanotubes Graphene oxide Cytotoxicity Genotoxicity
ISSN号	0169-1317
DOI	10.1016/j.clay.2021.106041
英文摘要	This study aimed at comparative examining of the interactions between conventionally used clay and carbon nanomaterials and human lung adenocarcinoma cells (A549 cells). The following platy and tubular nanomaterials were tested: carbon nanoparticles, i.e. multi-walled carbon nanotubes (MWCNTs) and graphene oxide nanosheets (GO) as well as nanoclays, i.e. halloysite nanotubes (HNTs) and kaolinite nanosheets (Kaol). Nanoparticle physicochemical properties and their internalization into cells were examined using dynamic light scattering as well as atomic force, 3D laser scanning confocal and darkfield hyperspectral microscopies. Biological aspects of the nanomaterial-cell interaction included assessment of cellular toxicity, DNA damage, metabolic activity, and physical parameters of the cells. Regardless of a shape, carbon nanomaterials demonstrated cell surface adsorption, but negligible penetration into cells compared to nanoclays. However, carbon nanomaterials were found to be the most toxic for cells as probed by the MTS assay. They also turned out to be the most genotoxic for cells compared to nanoclays as revealed by the DNA-Comet assay. GO significantly increased the fraction of apoptotic cells and was the most cytotoxic and genotoxic nanomaterial. Comparison of flow cytometry and MTS data indicated that a cytotoxic effect of MWCNTs was not associated with increased cell death, but was rather due to a decrease in cell metabolic activity and/or proliferation. Finally, no significant effect of the shape of the tested nanomaterials on their internalization and cytotoxicity was revealed.
资助项目	RFBR[18-53-80067 BRICS_t] ; National Natural Science Fund BRICS STI Framework Program of China[51861145304]
WOS关键词	DNA-DAMAGE ; IN-VITRO ; OXIDATIVE STRESS ; CELLULAR TOXICITY ; COMET ASSAY ; NANOPARTICLES ; GENOTOXICITY ; DELIVERY ; SURFACE ; ADSORPTION
WOS研究方向	Chemistry ; Materials Science ; Mineralogy
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000647564700006
资助机构	RFBR ; National Natural Science Fund BRICS STI Framework Program of China
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/48778]
专题	中国科学院过程工程研究所
通讯作者	Fakhrullin, Rawil
作者单位	1.Kazan Fed Univ, Inst Fundamental Med & Biol, Kreml Uram 18, Kazan 420008, Republic Of Tat, Russia 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Nottingham Trent Univ, Sch Sci & Technol, Dept Chem & Forens, Clifton Lane, Nottingham NG11 8NS, England 4.Fraunhofer Inst Cell Therapy & Immunol, Branch Bioanalyt & Bioproc, Muhlenberg 13, D-14476 Potsdam, Germany
推荐引用方式 GB/T 7714	Rozhina, Elvira,Batasheva, Svetlana,Miftakhova, Regina,et al. Comparative cytotoxicity of kaolinite, halloysite, multiwalled carbon nanotubes and graphene oxide[J]. APPLIED CLAY SCIENCE,2021,205:10.
APA	Rozhina, Elvira.,Batasheva, Svetlana.,Miftakhova, Regina.,Yan, Xuehai.,Vikulina, Anna.,...&Fakhrullin, Rawil.(2021).Comparative cytotoxicity of kaolinite, halloysite, multiwalled carbon nanotubes and graphene oxide.APPLIED CLAY SCIENCE,205,10.
MLA	Rozhina, Elvira,et al."Comparative cytotoxicity of kaolinite, halloysite, multiwalled carbon nanotubes and graphene oxide".APPLIED CLAY SCIENCE 205(2021):10.