With the increasing interest on the development of electrochemical techniques in multi-fields, it is highly desirable to explore a dual-functional electrochemical sensor for medical diagnosis and environmental monitoring. In this work, the tin-doped indium sulfide 2D nanomaterial was synthesized via a solvothermal method followed by thermal calcination. Due to the enhanced charge transfer and efficient metal active center, the Sn-In2S3 2D sensing interface exhibits the excellent electrochemical performance on uric acid (UA) and Pb(II) detection. It offers a satisfied detection range of 20-1500 mu M towards UA with the favorable oxidation potential of 0.1 V based on the SWV technique, while the detection limit can be evaluated as 0.62 mu M. On the other hand, a high sensitivity of 671.9 mu A mu M-1 cm-2 with a detection limit of 0.024 mu M is obtained for the reduction and stripping of Pb(II). Moreover, the practical application of the electrochemical detection of UA in human urine and Pb(II) in Taihu Lake samples were evaluated with the high reliability and good recovery. The as-prepared tin-doped indium sulfide 2D nanomaterial showed the great application potential as the dual-functional sensing platform for electrochemical sensing.