Rapid and simple detection ofBacillus cereusin milk by real-time competitive annealing mediated isothermal amplification

doi:10.1039/d0an00965b

	Rapid and simple detection ofBacillus cereusin milk by real-time competitive annealing mediated isothermal amplification
	Bao, Haiyan 2; Li, Wei 4; Yue, Xiqing 2; Wu, Junrui 2; Qiao, Yu 1; Peng, Qing 1; Shi, Bo 1; Du, Yuguang 3; Chen, Xu 2; Wu, Rina 2
刊名	ANALYST
	2020-10-21
卷号	145 期号:20 页码:6677-6682
ISSN号	0003-2654
DOI	10.1039/d0an00965b
英文摘要	Bacillus cereus(B. cereus) is widespread in nature and considered an important foodborne pathogen, which can lead to emetic syndrome and diarrheal illness. Therefore, appropriate detection methods are needed to effectively monitor this pathogenic bacterium. Competitive annealing mediated isothermal amplification (CAMP) is a novel nucleic-acid-based detection technology that amplifies DNA with high sensitivity and specificity under isothermal conditions. The aim of this study was to develop a real-time CAMP assay for the rapid and simple detection ofB. cereusin milk. In this system, a pair of primers was designed to specifically target theentFMgene ofB. cereus. Compared with the conventional PCR method, the CAMP assay has higher sensitivity, the same specificity and shorter detection time. The detection limits of the CAMP assay for pure bacterial cultures and artificially contaminated milk samples were all 59 CFU mL(-1). And this detection method showed a wide linear range (from 5.9 x 10(5)to 59 CFU mL(-1)) and satisfactory recovery values (from 75.76% to 106.78%). These results indicate that the developed CAMP assay is a potentially useful method for the detection ofB. cereusin milk.
资助项目	National Key R D Project[2018YFD0501403] ; Youth Project of the Education Department of Liaoning Province, China[LSNQN201911]
WOS关键词	EMETIC BACILLUS-CEREUS ; ENTEROTOXIN GENES ; PROPIDIUM MONOAZIDE ; FOOD ; PCR ; STRAINS ; DIFFERENTIATION ; QUANTIFICATION ; ELISA ; ASSAY
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000576736000027
资助机构	National Key R D Project ; Youth Project of the Education Department of Liaoning Province, China
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/42330]
专题	中国科学院过程工程研究所
通讯作者	Chen, Xu; Wu, Rina
作者单位	1.Chinese Acad Agr Sci, Feed Res Inst, Beijing 10008, Peoples R China 2.Shenyang Agr Univ, Food Sci Coll, Shenyang 110866, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China 4.Shenyang Agr Univ, Dept Publicat, Shenyang 110866, Peoples R China
推荐引用方式 GB/T 7714	Bao, Haiyan,Li, Wei,Yue, Xiqing,et al. Rapid and simple detection ofBacillus cereusin milk by real-time competitive annealing mediated isothermal amplification[J]. ANALYST,2020,145(20):6677-6682.
APA	Bao, Haiyan.,Li, Wei.,Yue, Xiqing.,Wu, Junrui.,Qiao, Yu.,...&Wu, Rina.(2020).Rapid and simple detection ofBacillus cereusin milk by real-time competitive annealing mediated isothermal amplification.ANALYST,145(20),6677-6682.
MLA	Bao, Haiyan,et al."Rapid and simple detection ofBacillus cereusin milk by real-time competitive annealing mediated isothermal amplification".ANALYST 145.20(2020):6677-6682.