Excellent Protein Immobilization and Stability on Heterogeneous C-TiO2 Hybrid Nanostructures: A Single Protein AFM Study

doi:10.1021/acs.langmuir.0c01942

	Excellent Protein Immobilization and Stability on Heterogeneous C-TiO2 Hybrid Nanostructures: A Single Protein AFM Study
	Dong, Yihui 2; Ji, Xiaoyan 1; Laaksonen, Aatto 2,3,4; Cao, Wei 5; He, Hongyan 6; Lu, Xiaohua 2
刊名	LANGMUIR
	2020-08-11
卷号	36 期号:31 页码:9323-9332
ISSN号	0743-7463
DOI	10.1021/acs.langmuir.0c01942
英文摘要	Enhancing molecular interaction is critical for improving the immobilization and stability of proteins on TiO2 surfaces. In this work, mesoporous TiO2 materials with varied pore geometries were decorated with phenyl phosphoric acid (PPA), followed by a thermal treatment to obtain chemically heterogeneous C-TiO2 samples without changing the geometry and crystalline structure, which can keep the advantages of both carbon and TiO2. The molecular interaction force between the protein and the surfaces was measured using atomic force microscopy by decomposing from the total adhesion forces, showing that the surface chemistry determines the interaction strength and depends on the amount of partial carbon coverage on the TiO2 surface (similar to 40-80%). Samples with 58.3% carbon coverage provide the strongest molecular interaction force, consistent with the observation from the detected friction force. Surface-enhanced Raman scattering and electrochemical biosensor measurements for these C-TiO2 materials were further conducted to illustrate their practical implications, implying their promising applications such as in protein detection and biosensing.
资助项目	National Natural Science Foundation of China[21838004] ; National Natural Science Foundation of China[21776278] ; Ministry of Research and Innovation of Romania, CNCS.UEFISCDI within PNCDI III[PN-III-P4-ID-PCCF-2016-0050] ; Sackler Center for Computational Molecular and Materials Science at Tel Aviv University ; Foreign Postdoctoral Fellowship Program of the Israel Academy of Sciences and Humanities
WOS关键词	TITANIUM-DIOXIDE ; SURFACE-CHEMISTRY ; CARBON NANOTUBES ; PORE-SIZE ; TIO2 ; ADSORPTION ; PERFORMANCE ; ADHESION ; NANOCOMPOSITE ; BIOMATERIALS
WOS研究方向	Chemistry ; Materials Science
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000562137700032
资助机构	National Natural Science Foundation of China ; Ministry of Research and Innovation of Romania, CNCS.UEFISCDI within PNCDI III ; Sackler Center for Computational Molecular and Materials Science at Tel Aviv University ; Foreign Postdoctoral Fellowship Program of the Israel Academy of Sciences and Humanities
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/41766]
专题	中国科学院过程工程研究所
通讯作者	Ji, Xiaoyan; Lu, Xiaohua
作者单位	1.Lulea Univ Technol, Div Energy Sci, Energy Engn, S-97187 Lulea, Sweden 2.Nanjing Tech Univ, State Key Lab Mat Oriented Chem Engn, Nanjing 210009, Peoples R China 3.Stockholm Univ, Dept Mat & Environm Chem, Arrhenius Lab, SE-10691 Stockholm, Sweden 4.Petru Poni Inst Macromol Chem, Ctr Adv Res Bionanoconjugates & Biopolymers, Iasi 700487, Romania 5.Tel Aviv Univ, Sch Chem, IL-69978 Tel Aviv, Israel 6.Chinese Acad Sci, Inst Proc Engn, Beijing Key Lab Ion Liquids Clean Proc, CAS Key Lab Green Proc & Engn,State Key Lab Multi, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Dong, Yihui,Ji, Xiaoyan,Laaksonen, Aatto,et al. Excellent Protein Immobilization and Stability on Heterogeneous C-TiO2 Hybrid Nanostructures: A Single Protein AFM Study[J]. LANGMUIR,2020,36(31):9323-9332.
APA	Dong, Yihui,Ji, Xiaoyan,Laaksonen, Aatto,Cao, Wei,He, Hongyan,&Lu, Xiaohua.(2020).Excellent Protein Immobilization and Stability on Heterogeneous C-TiO2 Hybrid Nanostructures: A Single Protein AFM Study.LANGMUIR,36(31),9323-9332.
MLA	Dong, Yihui,et al."Excellent Protein Immobilization and Stability on Heterogeneous C-TiO2 Hybrid Nanostructures: A Single Protein AFM Study".LANGMUIR 36.31(2020):9323-9332.