The Pneumococcal Polysaccharide-Tetanus Toxin Native C-Fragment Conjugate Vaccine: The Carrier Effect and Immunogenicity

doi:10.1155/2020/9596129

	The Pneumococcal Polysaccharide-Tetanus Toxin Native C-Fragment Conjugate Vaccine: The Carrier Effect and Immunogenicity
	Yu, Rui 1; Xu, Junjie 1; Hu, Tao 2; Chen, Wei 1
刊名	MEDIATORS OF INFLAMMATION
	2020-07-04
卷号	2020 页码:11
ISSN号	0962-9351
DOI	10.1155/2020/9596129
英文摘要	The encapsulated bacteria, asStreptococcus pneumonia,Haemophilus influenzaetype b, andNeisseria meningitidis, cause serious morbidity and mortality worldwide. The capsular polysaccharide (PS), which could elicit a weak T cell-independent immune response, is a vital virulence determinant. One of the strategies to improve the PS-specific immunogenicity is to conjugate PS with a nontoxic carrier protein. Tetanus toxoid (TT) and CRM197 are the typical carrier proteins for the PS conjugate vaccines. TT is the inactivated tetanus toxin manipulated with formaldehyde, which suffers from the pollution from residual formaldehyde and the incomplete detoxification. CRM197 has the disadvantage of low-yield purification with the requirement of sophisticated culture conditions. Thus, a novel carrier protein without these disadvantages is highly required. The tetanus toxin native C-fragment (Hc) is safe, low-cost, and highly immunogenic with easy purification, which can act as a promising carrier protein. Pneumococcal serogroups 14 and 23F were major epidemic causes of pneumococcal infections. In the present study, the capsular PSs (PS14 and PS23F) were conjugated with Hc, TT, and CRM197, respectively. TT- and CRM197-based conjugates acted as controls for Hc-based conjugates (PS14-Hc and PS23F-Hc). The structural properties of Hc were not fundamentally changed after conjugated with PS. PS14-Hc and PS23F-Hc could potentiate sound PS-specific antibody levels comparable to the controls. Thus, Hc exhibited a practical carrier effect to help the pneumococcal conjugate vaccines perform good immunogenicities.
资助项目	National Key Research and Development Project of China[2018YFA0900804] ; National Natural Science Foundation of China[31970875] ; National Natural Science Foundation of China[81703445]
WOS关键词	PROTEIN CARRIERS ; IMPROVES ; DISEASE
WOS研究方向	Cell Biology ; Immunology
语种	英语
出版者	HINDAWI LTD
WOS记录号	WOS:000552753300001
资助机构	National Key Research and Development Project of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/41591]
专题	中国科学院过程工程研究所
通讯作者	Hu, Tao; Chen, Wei
作者单位	1.Beijing Inst Biotechnol, Lab Vaccine & Antibody Engn, Beijing 100071, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Yu, Rui,Xu, Junjie,Hu, Tao,et al. The Pneumococcal Polysaccharide-Tetanus Toxin Native C-Fragment Conjugate Vaccine: The Carrier Effect and Immunogenicity[J]. MEDIATORS OF INFLAMMATION,2020,2020:11.
APA	Yu, Rui,Xu, Junjie,Hu, Tao,&Chen, Wei.(2020).The Pneumococcal Polysaccharide-Tetanus Toxin Native C-Fragment Conjugate Vaccine: The Carrier Effect and Immunogenicity.MEDIATORS OF INFLAMMATION,2020,11.
MLA	Yu, Rui,et al."The Pneumococcal Polysaccharide-Tetanus Toxin Native C-Fragment Conjugate Vaccine: The Carrier Effect and Immunogenicity".MEDIATORS OF INFLAMMATION 2020(2020):11.