Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction

doi:10.3389/fbioe.2020.00272

	Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction
	Jing, Bolin 1,2; Wang, Zhuo A.1; Zhang, Chen 1; Deng, Quanfeng 3; Wei, Jinhua 1; Luo, Yong 3; Zhang, Xiuli 4; Li, Jianjun 1; Du, Yuguang 1
刊名	FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
	2020-03-31
卷号	8 页码:14
关键词	peristaltic microfluidic chip human gut-vessel co-culture model host-microbial interaction Lactobacillus casei enteritis injury
ISSN号	2296-4185
DOI	10.3389/fbioe.2020.00272
英文摘要	Intestinal floras influence a lot of biological functions of the organism. Although animal model are strong tools for researches on the relationship between host and microbe, a physiologically relevant in vitro human gut model was still required. Here, a novel human gut-vessel microfluidic system was established to study the host-microbial interaction. Peristaltic motion of the cells on the chip was driven by a pneumatic pump. When intestinal epithelial cells (Caco2) were co-cultured with vascular endothelial cells (HUVECs) on the peristaltic microfluidic chip, Caco2 showed normal barrier and absorption functions after 5 days cultivation, which generally took 21 days in static Transwell models. Intestinal microvilli and glycocalyx layer were seen after 4 days cultivation, and Lactobacillus casei was successfully co-cultured for a week in the intestinal cavity. A model for intestinal damage and inflammatory responses caused by E. coli was set up on this chip, which were successfully suppressed by Lactobacillus casei or antibiotic. In summary, this human gut-vessel microfluidic system showed a good potential for investigating the host-microbial interaction and the effect and mechanism of microbiome on intestinal diseases in vitro.
资助项目	National Natural Science Foundation of China[31870802] ; National Natural Science Foundation of China[31700705]
WOS关键词	INFLAMMATORY-BOWEL-DISEASE ; DRUG PERMEABILITY ; ENDOTHELIAL-CELLS ; STEM-CELLS ; CACO-2 ; LACTOBACILLI ; MODEL ; DIFFERENTIATION ; BIFIDOBACTERIA ; BACTERIA
WOS研究方向	Biotechnology & Applied Microbiology ; Science & Technology - Other Topics
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000526743400001
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/40110]
专题	中国科学院过程工程研究所
通讯作者	Zhang, Xiuli; Li, Jianjun; Du, Yuguang
作者单位	1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Dept Chem, Beijing, Peoples R China 3.Dalian Univ Technol, Dept Chem Engn, Key Lab Fine Chem, Dalian, Peoples R China 4.Soochow Univ, Coll Pharmaceut Sci, Suzhou, Peoples R China
推荐引用方式 GB/T 7714	Jing, Bolin,Wang, Zhuo A.,Zhang, Chen,et al. Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2020,8:14.
APA	Jing, Bolin.,Wang, Zhuo A..,Zhang, Chen.,Deng, Quanfeng.,Wei, Jinhua.,...&Du, Yuguang.(2020).Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,8,14.
MLA	Jing, Bolin,et al."Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 8(2020):14.