A new transcription factor ATG10S activates IFNL2 transcription by binding at an IRF1 site in HepG2 cells | |
Zhang, Miao-Qing1,2,3; Zhao, Qiong2; Zhang, Jing-Pu2 | |
刊名 | AUTOPHAGY |
2020-01-31 | |
页码 | 13 |
关键词 | Atg10s Core Motif Functional Nucleotides Ifnl2 Promoter Ifnl2 Transcription Irf1 |
ISSN号 | 1554-8627 |
DOI | 10.1080/15548627.2020.1719681 |
英文摘要 | IFNL2 is a potent antiviral interferon, but the regulation of its gene expression is not fully clear. Here, we report the regulation of ATG10S for IFNL2 transcription. Through sequential deletion of the IFNL2 promoter sequence, we found LP1-1, a fragment of the promoter responding to ATG10S activity. Subcellular localization and DNA immunoprecipitation assays showed ATG10S translocating into the nucleus and binding to LP1-1. Online prediction for transcription factor binding sites showed an IRF1 targeting locus in LP1-1. Luciferase assays, RT-PCR, and western blot analysis revealed a core motif (CAAGAC) existing in LP1-1, which determined ATG10S and IRF1 activity; individual nucleotide substitution showed that the functional nucleotides of ATG10S targeting were C1, A3, and C6, and the ones associated with IRF1 were A3 and G4 within the core motif. Co-immunoprecipitation assays revealed ATG10S combination with KPNA1/importin alpha, KPNB1/importin beta, and IRF1. The knockdown of endogenous IRF1 increased ATG10S activity on IFNL2 transcription. These results indicate that ATG10S as a transcription factor competes with IRF1 for the same binding site to promote IFNL2 gene transcription. |
资助项目 | National Natural Science Foundation of China[81373453] ; Chinese National Key Technology RD Program[2015BAK45B01] ; Foundation for Innovative Research Groups of the National Natural Science Foundation of China[81621064] |
WOS关键词 | Interferon-lambda ; Virus-replication ; Nuclear Import ; Hepatitis-b ; Recognition ; Autophagy ; Family ; Regulator ; Pathways ; Reveals |
WOS研究方向 | Cell Biology |
语种 | 英语 |
出版者 | TAYLOR & FRANCIS INC |
WOS记录号 | WOS:000511751400001 |
资助机构 | National Natural Science Foundation of China ; Chinese National Key Technology RD Program ; Foundation for Innovative Research Groups of the National Natural Science Foundation of China |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/39321] |
专题 | 中国科学院过程工程研究所 |
通讯作者 | Zhang, Jing-Pu |
作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Postdoctoral Mobile Res Stn, Beijing, Peoples R China 2.Chinese Acad Med Sci & Peking Union Med Coll, Key Lab Biotechnol Antibiot, Inst Med Biotechnol, Beijing Key Lab Antimicrobial Agents,NHC, Beijing 100050, Peoples R China 3.China Resources Sanjiu Med & Pharmaceut Co Ltd, Postdoctoral Sci Res Workstn, Shenzhen, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Miao-Qing,Zhao, Qiong,Zhang, Jing-Pu. A new transcription factor ATG10S activates IFNL2 transcription by binding at an IRF1 site in HepG2 cells[J]. AUTOPHAGY,2020:13. |
APA | Zhang, Miao-Qing,Zhao, Qiong,&Zhang, Jing-Pu.(2020).A new transcription factor ATG10S activates IFNL2 transcription by binding at an IRF1 site in HepG2 cells.AUTOPHAGY,13. |
MLA | Zhang, Miao-Qing,et al."A new transcription factor ATG10S activates IFNL2 transcription by binding at an IRF1 site in HepG2 cells".AUTOPHAGY (2020):13. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论