Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus

doi:10.1016/j.chroma.2019.460578

	Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus
	An, Ning 2; Gong, Pisheng 2; Hou, Henglei 2; Chi, Weiya 2; Jin, Haibo 2; Zhao, Lan 1; Tan, Qiqi 2; Tang, Xu 2; Wang, Fei 2; Jin, Hongchao 2
刊名	JOURNAL OF CHROMATOGRAPHY A
	2020-01-11
卷号	1610 页码:4
关键词	Negative chromatography Macroporous microsphere with core-shell structure Purification of virus Avian influenza virus
ISSN号	0021-9673
DOI	10.1016/j.chroma.2019.460578
英文摘要	The macroporous microspheres with core-shell structure, based on a copolymer of 4-Vinylbenzyl chloride, glycidyl methacrylate, and ethylene glycol dimethacrylate, were fabricated through atom transfer radical polymerization suspension polymerization. The microspheres showed 100-200 nm pores in shell and 500-900 nm pores in core. The shell was hydrophilic modified through grafting of poly(N-hydroxyethyl acrylamide) onto the shell surface for reducing adsorption of proteins. The core was coupled with a ligand of poly(ethylene imine) that could bind the proteins. Feedstock of avian influenza virus could be purified on these modified microspheres through negative chromatography. Avian influenza virus cannot enter the core and was recovered from the flow-through, while other proteins with negative charges were able to penetrate into the core and bind to the poly(ethylene imine) ligands. The dynamic binding capacity of proteins was higher on this medium (61 mg/mL) than the commercially available resin (12 mg/mL, Capto Core 700). (C) 2019 Elsevier B.V. All rights reserved.
资助项目	Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality[IDHT20180508] ; Beijing Natural Science Foundation[2162013] ; Beijing Natural Science Foundation[2172054] ; Construction of Scientific Research Platform[2018XK002]
WOS关键词	PROTEIN
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000509816200042
资助机构	Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality ; Beijing Natural Science Foundation ; Construction of Scientific Research Platform
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/39285]
专题	中国科学院过程工程研究所
通讯作者	Zhang, Rongyue
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Beijing Inst Petrochem Technol, Beijing Key Lab Fuels Cleaning & Adv Catalyt Emis, Coll Chem Engn, Beijing 102617, Peoples R China
推荐引用方式 GB/T 7714	An, Ning,Gong, Pisheng,Hou, Henglei,et al. Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus[J]. JOURNAL OF CHROMATOGRAPHY A,2020,1610:4.
APA	An, Ning.,Gong, Pisheng.,Hou, Henglei.,Chi, Weiya.,Jin, Haibo.,...&Zhang, Rongyue.(2020).Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus.JOURNAL OF CHROMATOGRAPHY A,1610,4.
MLA	An, Ning,et al."Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus".JOURNAL OF CHROMATOGRAPHY A 1610(2020):4.