Sensitive and selective fluorometric determination of DNA by using layered hexagonal nanosheets of a covalent organic framework prepared from p-phenylenediamine and benzene-1,3,5-tricarboxaldehyde | |
Mandal, Tapas Kumar1; Parvin, Nargish2; Mishra, Kanchan1; Mohandoss, Sonaimuthu1; Lee, Yong Rok1 | |
刊名 | MICROCHIMICA ACTA |
2019-12-01 | |
卷号 | 186期号:12页码:8 |
关键词 | Porous nanomaterials Bond Fluorescence Signal Target Biomolecules Biomarker Diseases Sensor Diagnostics |
ISSN号 | 0026-3672 |
DOI | 10.1007/s00604-019-3944-1 |
英文摘要 | A modified method is described for the preparation of amino-functionalized covalent organic framework nanosheets (COF-NSs). These consist of hexagonal layered sheets and were prepared from commercially available starting materials (p-phenylenediamine and benzene-1,3,5-tricarboxaldehyde). The interlayer stacking interactions between the ultra-thin COF-NSs became weak because the pi stacking is destroyed by sonication. This result in the exfoliation of COF-NSs. As an application, the COF-NSs used for sensitive and selective fluorometric determination of DNA. To reach this goal, H1 and H2 hairpin-like DNA probes were chosen; H1 used Texas Red-labeled dye as a fluorescent probe. The addition of the COF-NSs, the hairpin probes was adsorbed onto the porous surface of the COFNSs. The pi stacking and hydrogen-bond interactions between COFNSs and nucleic acid quench the fluorescence of the Texas red-labeled probe. The target DNA enables the recovery of the quenched fluorescence of the Texas red-labelled probe by triggering an inter-chain hybridization within hairpin probes. This results in a weaker interaction of double-stranded DNA (dsDNA) with the COFNSs. Consequently, the dsDNA detaches from the COFNSs, thereby recovering the dye's fluorescence (excitation/emission maxima at 590/612 nm) with increasing target DNA concentration. The findings were applied to design a method for the determination of DNA that has a 2 pM detection limit. This is significantly lower than the limit of detection reported previously for 2D nanomaterial-based fluorometric DNA assays. |
资助项目 | National Research Foundation of Korea (NRF) - Korea government (MSIT)[2018R1A2B2004432] |
WOS关键词 | GRAPHENE OXIDE |
WOS研究方向 | Chemistry |
语种 | 英语 |
出版者 | SPRINGER WIEN |
WOS记录号 | WOS:000499967800017 |
资助机构 | National Research Foundation of Korea (NRF) - Korea government (MSIT) |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/38339] |
专题 | 中国科学院过程工程研究所 |
通讯作者 | Mandal, Tapas Kumar |
作者单位 | 1.Yeungnam Univ, Sch Chem Engn, Gyongsan 38541, South Korea 2.Chinese Acad Sci, Inst Proc Engn, CAS Ctr Excellence Nanosci, State Key Lab Biochem Engn, 1 Beiertiao, Beijing 100190, Peoples R China |
推荐引用方式 GB/T 7714 | Mandal, Tapas Kumar,Parvin, Nargish,Mishra, Kanchan,et al. Sensitive and selective fluorometric determination of DNA by using layered hexagonal nanosheets of a covalent organic framework prepared from p-phenylenediamine and benzene-1,3,5-tricarboxaldehyde[J]. MICROCHIMICA ACTA,2019,186(12):8. |
APA | Mandal, Tapas Kumar,Parvin, Nargish,Mishra, Kanchan,Mohandoss, Sonaimuthu,&Lee, Yong Rok.(2019).Sensitive and selective fluorometric determination of DNA by using layered hexagonal nanosheets of a covalent organic framework prepared from p-phenylenediamine and benzene-1,3,5-tricarboxaldehyde.MICROCHIMICA ACTA,186(12),8. |
MLA | Mandal, Tapas Kumar,et al."Sensitive and selective fluorometric determination of DNA by using layered hexagonal nanosheets of a covalent organic framework prepared from p-phenylenediamine and benzene-1,3,5-tricarboxaldehyde".MICROCHIMICA ACTA 186.12(2019):8. |
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