Comparative toxicological effects of planktonic Microcystis and benthic Oscillatoria on zebrafish embryonic development: Implications for cyanobacteria risk assessment

	Comparative toxicological effects of planktonic Microcystis and benthic Oscillatoria on zebrafish embryonic development: Implications for cyanobacteria risk assessment
	Li, Hongmin; Gu, Xiaohong; Chen, Huihui; Mao, Zhigang; Zeng, Qingfei; Yang, Huiting; Kan, Kecong
刊名	ENVIRONMENTAL POLLUTION
	2021
卷号	274
英文摘要	Planktonic and benthic cyanobacteria blooms are increasing in frequency in recent years. Although many studies have focused on the effects of purified toxins or cyanobacteria extracts on fish developments, the more complex impacts of cyanobacteria cells on fish populations are still considered insufficient. This study compared the toxicological effects of harmful planktonic Microcystis and benthic Oscillatoria on zebrafish (Danio rerio) early stages of development. Zebrafish embryos, at 1-2 h post fertilization (hpf), were exposed to 5, 10, and 20 x 10(5) cells/mL Microcystis (producing microcystins) or Oscillatoria (producing cylindrospermopsins) until 96 hpf. The results indicated that the effects of benthic Oscillatoria on embryonic development of zebrafish were different from those of planktonic Microcystis. Reduced hatching rates, increased mortality, depressed heart rates and elevated malformation rates were observed following exposures to increased concentrations of Microcystis, whilst Oscillatoria exposures only caused yolk sac edemas. Exposure to a high concentration of Microcystis induced severe oxidative damage, growth inhibition and transcriptional downregulations of genes (GH, GHR1, IGF1, IGF1rb) associated with the growth hormone/insulin-like growth factor (GH/IGF) axis. Although Oscillatoria exposure did not affect the body growth, it obviously enhanced the antioxidant enzyme activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) and up-regulated the expressions of several oxidative stress-related genes. Discrepancies in the developmental toxicity caused by Microcystis and Oscillatoria may not only attributed to the different secondary metabolites they secrete, but also to the different adhesion behaviors of algal cells on embryonic chorion. These results suggested that harmful cyanobacteria cells could influence the successful recruitment of fish, while the effects of benthic cyanobacteria should not be ignored. It also highlighted that the necessity for further investigating the ecotoxicity of intact cyanobacterial samples when assessing the risk of cyanobacterial blooms. (C) 2020 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://159.226.73.51/handle/332005/20583]
专题	中国科学院南京地理与湖泊研究所
推荐引用方式 GB/T 7714	Li, Hongmin,Gu, Xiaohong,Chen, Huihui,et al. Comparative toxicological effects of planktonic Microcystis and benthic Oscillatoria on zebrafish embryonic development: Implications for cyanobacteria risk assessment[J]. ENVIRONMENTAL POLLUTION,2021,274.
APA	Li, Hongmin.,Gu, Xiaohong.,Chen, Huihui.,Mao, Zhigang.,Zeng, Qingfei.,...&Kan, Kecong.(2021).Comparative toxicological effects of planktonic Microcystis and benthic Oscillatoria on zebrafish embryonic development: Implications for cyanobacteria risk assessment.ENVIRONMENTAL POLLUTION,274.
MLA	Li, Hongmin,et al."Comparative toxicological effects of planktonic Microcystis and benthic Oscillatoria on zebrafish embryonic development: Implications for cyanobacteria risk assessment".ENVIRONMENTAL POLLUTION 274(2021).