Clostridium prolinivorans sp. nov., a thermophilic bacterium isolated from an anaerobic reactor degrading propionate

	Clostridium prolinivorans sp. nov., a thermophilic bacterium isolated from an anaerobic reactor degrading propionate
	Huang, Yan; Wei, Zhixian; Cong, Lulu; Qiu, Zhongwei; Chen, Rui; Deng, Yu; Zhang, Yinggang; Fan, Hui; Ma, Shichun
刊名	INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
	2020
卷号	70 期号:10 页码:5190-5196
英文摘要	An anaerobic, Gram-stain-positive, spore-forming bacterium, designated strain PYR-10(T), was isolated from a mesophilic methanogenic consortium. Cells were 0.7-1.2 x 6.0-6.3 mu m, straight or slightly curved rods, with flagellar motility. Growth was observed in PYG (peptone-yeast glucose) medium at pH 5.5-8.0 (optimum, pH 6.5), 30-55 degrees C (45 degrees C) and in NaCl concentrations of 0-15 g l(-1) (0 g l(-1)). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain PYR-10(T) belongs to the genus Clostridium. The strain showed 95.4, 93.7, 93.5 and 93.0% 16S rRNA gene sequence similarity to Clostridium swellfunianum DSM 27788(T), Clostridium pascui DSM 10365(T), Clostridium pasteurianum DSM 525(T) and Clostridium punense DSM 28650(T), respectively. The genomic DNA G+C content was 27.7 mol%. The major cellular fatty acids of strain PYR-10(T) were iso-C-15:0, C-16:0, C-16:0 DMA, anteiso-C-15:0 and C-14:0. The main polar lipids were glycolipid, phosphoaminoglycolipid, diphosphatidylglycerol, phosphatidylglycerol, phospholipids, phosphatidylethanolamine and lipids. An unknown menaquinone was detected. 2,6-Diaminopimelic acid was not detected. The whole-cell sugars contained ribose and lower amounts of glucose. Based on the results of phylogenetic, chemotaxonomic and phenotypic analyses, strain PYR-10(T) represents a novel species of the genus Clostridium, for which the name Clostridium prolinivorans sp. nov. is proposed. The type strain is strain PYR-10(T) (=JCM 33161(T) =CCAM 531(T)=CGMCC 1.5286(T)).
内容类型	期刊论文
源URL	[http://159.226.73.51/handle/332005/20049]
专题	中国科学院南京地理与湖泊研究所
推荐引用方式 GB/T 7714	Huang, Yan,Wei, Zhixian,Cong, Lulu,et al. Clostridium prolinivorans sp. nov., a thermophilic bacterium isolated from an anaerobic reactor degrading propionate[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2020,70(10):5190-5196.
APA	Huang, Yan.,Wei, Zhixian.,Cong, Lulu.,Qiu, Zhongwei.,Chen, Rui.,...&Ma, Shichun.(2020).Clostridium prolinivorans sp. nov., a thermophilic bacterium isolated from an anaerobic reactor degrading propionate.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,70(10),5190-5196.
MLA	Huang, Yan,et al."Clostridium prolinivorans sp. nov., a thermophilic bacterium isolated from an anaerobic reactor degrading propionate".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 70.10(2020):5190-5196.