A homogeneous time-resolved fluorometric energy transfer assay for the binding assessment of FcRn with IgG antibodies

doi:10.1016/j.jim.2021.113180

	A homogeneous time-resolved fluorometric energy transfer assay for the binding assessment of FcRn with IgG antibodies
	Song, Yakai 2; Deng, Xiaojie 2; Shi, Wei 1; Tang, Feng 1; Huang, Wei 1; Gong, Likun 2; Qin, Qiuping 2
刊名	JOURNAL OF IMMUNOLOGICAL METHODS
	2021-12-01
卷号	499 页码:6
关键词	FcRn TR-FRET mAb ADC Pharmacokinetics
ISSN号	0022-1759
DOI	10.1016/j.jim.2021.113180
通讯作者	Gong, Likun(lkgong@cdser.simm.ac.cn) ; Qin, Qiuping(qpqin@cdser.simm.ac.cn)
英文摘要	We aimed to develop a homogeneous time-resolved fluorometric energy transfer assay for assessment of human neonatal Fc receptor binding activity with IgG-type antibodies. The assay was configured with FcRn-coupled with Eu cryptate via biotin and streptavidin interaction as donor and IgG1 labeled with d2 as acceptor. Only a single incubation step was involved and no wash step was required. The assay demonstrated good accuracy, precision, linearity and specificity. Our further investigation with a rat pharmacokinetics study revealed that the terminal t1/2 for Trastuzumab and its related three ADCs agreed with the EC50 data. The assay can be applied to various IgGs with modifications to identify antibodies with appropriate binding ability to human FcRn.
资助项目	National Science & Technology Major Project 'Key New Drug Creation and Manufacturing Program' of China[2019ZX09732002-013]
WOS研究方向	Biochemistry & Molecular Biology ; Immunology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000720364300009
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/298952]
专题	中国科学院上海药物研究所
通讯作者	Gong, Likun; Qin, Qiuping
作者单位	1.Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Ctr Biotherapeut Discovery Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Ctr Drug Safety Evaluat & Res, Shanghai Inst Mat Med, Dept Immunoassay & Immunochem, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Song, Yakai,Deng, Xiaojie,Shi, Wei,et al. A homogeneous time-resolved fluorometric energy transfer assay for the binding assessment of FcRn with IgG antibodies[J]. JOURNAL OF IMMUNOLOGICAL METHODS,2021,499:6.
APA	Song, Yakai.,Deng, Xiaojie.,Shi, Wei.,Tang, Feng.,Huang, Wei.,...&Qin, Qiuping.(2021).A homogeneous time-resolved fluorometric energy transfer assay for the binding assessment of FcRn with IgG antibodies.JOURNAL OF IMMUNOLOGICAL METHODS,499,6.
MLA	Song, Yakai,et al."A homogeneous time-resolved fluorometric energy transfer assay for the binding assessment of FcRn with IgG antibodies".JOURNAL OF IMMUNOLOGICAL METHODS 499(2021):6.