Novel pectin-like polysaccharide from Panax notoginseng attenuates renal tubular cells fibrogenesis induced by TGF-beta

doi:10.1016/j.carbpol.2021.118772

	Novel pectin-like polysaccharide from Panax notoginseng attenuates renal tubular cells fibrogenesis induced by TGF-beta
	Huang, Chunfan 1,2,7; Jing, Xiaoqi 2,3; Wu, Qianhu 1,7; Ding, Kan 1,2,4,5,6
刊名	CARBOHYDRATE POLYMERS
	2022-01-15
卷号	276 页码:12
关键词	Panax notoginseng Polysaccharide Renal fibrosis Epithelial-mesenchymal transition Pectin
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2021.118772
通讯作者	Wu, Qianhu(jszjsjgb@163.com) ; Ding, Kan(dingkan@simm.ac.cn)
英文摘要	Renal fibrosis is the final common result of a variety of progressive injuries leading to chronic renal failure. However, there are no effective clinical available drugs for the treatment. Notoginsenoside from Panax notoginseng could ameliorate renal fibrosis. We hypothesized that polysaccharide from this herb might have similar bioactivity. Here, we elucidated structure of a novel pectin-like polysaccharide designed SQD4S2 with a netty antenna backbone of glucogalacturonan substituted by glucoarabinan, glucurogalactan and galactose residues from this herb. Interestingly, SQD4S2 could reverse the morphological changes of human renal tubular HK-2 cells induced by TGF-beta. Mechanism study suggested that this bioactivity might associate with N-cadherin (CDH2), Snail (SNAI1), Slug (SNAI2) depression and E-cadherin (CDH1) enhancement. In addition, SQD452 could impede critical fibrogenesis associated molecules such as alpha-SMA, fibronectin, vimentin, COL1A1, COL3A1, FN1 and ACTA2 expression induced by TGF-beta in HK-2 cells. Current findings outline a novel leading polysaccharide for against renal fibrosis new drug development.
资助项目	National Key Research and Development Program of China, Ministry of Science and Technology, China[2019YFC1711-000] ; Key New Drug Creation and Manufacturing Program[2019ZX09735001] ; Shanghai Municipal Science and Technology Major Project
WOS关键词	TO-MESENCHYMAL TRANSITION ; STRUCTURAL-CHARACTERIZATION ; FIBROSIS ; FLOWERS ; ARABINOGALACTAN ; ELUCIDATION ; MECHANISMS ; RESIDUE ; DAMAGE ; CKD
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000712456500007
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/298762]
专题	中国科学院上海药物研究所
通讯作者	Wu, Qianhu; Ding, Kan
作者单位	1.Nanjing Univ Chinese Med, Coll Pharm, 138 Xianlin Ave, Nanjing 210029, Jiangsu, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Medial, Lab Carbohydrate Based Drug Res Ctr, Glycochem & Glycobiol Lab, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China 3.Shanghai Univ Tradit Chinese Med, Inst Interdisciplinary Integrat Med Res, Shanghai 201203, Peoples R China 4.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 5.Henan Univ Chinese Med, Acad Chinese Med Sci, Henan Polysaccharide Res Ctr, Zhengzhou 450046, Henan, Peoples R China 6.Chinese Acad Sci, Shanghai Inst Mat Medial, Zhongshan Inst Drug Discovery, SSIP Healthcare & Med Demonstrat Zone, Zhongshan 528400, Guangdong, Peoples R China 7.Zhenjiang Third Peoples Hosp, 300 Daijiamen Ave, Zhenjiang 212021, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Huang, Chunfan,Jing, Xiaoqi,Wu, Qianhu,et al. Novel pectin-like polysaccharide from Panax notoginseng attenuates renal tubular cells fibrogenesis induced by TGF-beta[J]. CARBOHYDRATE POLYMERS,2022,276:12.
APA	Huang, Chunfan,Jing, Xiaoqi,Wu, Qianhu,&Ding, Kan.(2022).Novel pectin-like polysaccharide from Panax notoginseng attenuates renal tubular cells fibrogenesis induced by TGF-beta.CARBOHYDRATE POLYMERS,276,12.
MLA	Huang, Chunfan,et al."Novel pectin-like polysaccharide from Panax notoginseng attenuates renal tubular cells fibrogenesis induced by TGF-beta".CARBOHYDRATE POLYMERS 276(2022):12.