A Novel microRNA of Japanese Flounder Regulates Antimicrobial Immunity Involving a Bacteria-Binding CSF3

doi:10.3389/fimmu.2021.723401

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	A Novel microRNA of Japanese Flounder Regulates Antimicrobial Immunity Involving a Bacteria-Binding CSF3
	Li, Wenrui 1,2,3; Guan, Xiaolu 1,2; Sun, Bin 1,2,3; Sun, Li 1,2,3
刊名	FRONTIERS IN IMMUNOLOGY
	2021-08-19
卷号	12 页码:13
关键词	Paralichthys olivaceus fish microRNA granulocyte colony stimulating factor 3 bacterial and viral pathogens antimicrobial immune defense
ISSN号	1664-3224
DOI	10.3389/fimmu.2021.723401
通讯作者	Sun, Li(lsun@qdio.ac.cn)
英文摘要	MicroRNAs (miRNAs) are small non-coding RNAs that regulate diverse biological processes including immunity. In a previous high-throughput RNA sequencing study, a novel miRNA, pol-miR-novel_642, was identified from Japanese flounder (Paralichthys olivaceus), a farmed fish species with important economic value. In this study, we investigated the regulatory mechanism and the function of pol-miR-novel_642 and its target gene. We found that pol-miR-novel_642 targeted, in a sequence-specific manner, a flounder gene encoding an uncharacterized protein that is a structural homologue of murine granulocyte colony stimulating factor 3 (CSF3). The expression of pol-miR-novel_642 and its target gene (named PoCSF3-1) was regulated, in different manners, by the bacterial pathogen Edwardsiella tarda and the viral pathogen megalocytivirus. Overexpression of pol-miR-novel_642 or interference with PoCSF3-1 expression in flounder cells strongly potentiated E. tarda infection. Consistently, in vivo knockdown of PoCSF3-1 enhanced bacterial dissemination in flounder tissues but blocked viral replication, whereas in vivo overexpression of PoCSF3-1 inhibited bacterial dissemination and facilitated viral infection. Overexpression/knockdown of PoCSF3-1 and pol-miR-novel_642 also affected the activation of autophagy. Recombinant PoCSF3-1 (rPoCSF3-1) interacted with and inhibited the growth of Gram-negative bacteria in a manner relying on a PoCSF3-1-characteristic structural motif that is absent in mouse CSF3. rPoCSF3-1 also regulated the proliferation, inflammatory response, and immune defense of flounder head kidney leukocytes in a structure-dependent fashion. Together, these results reveal the function of a novel miRNA-CSF3 regulatory system of flounder, and add new insights into the role and mechanism of fish miRNA and CSF3 in antimicrobial immunity.
资助项目	National Natural Science Foundation of China[31730100] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Immunology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000693532600001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/176379]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Sun, Li
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Beijing, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Coll Marine Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Wenrui,Guan, Xiaolu,Sun, Bin,et al. A Novel microRNA of Japanese Flounder Regulates Antimicrobial Immunity Involving a Bacteria-Binding CSF3[J]. FRONTIERS IN IMMUNOLOGY,2021,12:13.
APA	Li, Wenrui,Guan, Xiaolu,Sun, Bin,&Sun, Li.(2021).A Novel microRNA of Japanese Flounder Regulates Antimicrobial Immunity Involving a Bacteria-Binding CSF3.FRONTIERS IN IMMUNOLOGY,12,13.
MLA	Li, Wenrui,et al."A Novel microRNA of Japanese Flounder Regulates Antimicrobial Immunity Involving a Bacteria-Binding CSF3".FRONTIERS IN IMMUNOLOGY 12(2021):13.