Mitochondrial proteomics reveals the mechanism of spermatogenic cells apoptosis induced by carbon ion radiation in zebrafish

doi:10.1002/jcp.28808

	Mitochondrial proteomics reveals the mechanism of spermatogenic cells apoptosis induced by carbon ion radiation in zebrafish
	Li, Hongyan 1,2,3,4; Zhang, Weihong 5; Zhang, Hong 1,2,3,4,6; Xie, Yi 1,2,3; Sun, Chao 1,2,3; Di, Cuixia 1,2,3; Si, Jing 1,2,3; Gan, Lu 1,2,3; Yan, Junfang 1,2,3,4
刊名	JOURNAL OF CELLULAR PHYSIOLOGY
	2019-12-01
卷号	234 期号:12 页码:22439-22449
关键词	carbon ion radiation mitochondria spermatogenic cells zebrafish
ISSN号	0021-9541
DOI	10.1002/jcp.28808
通讯作者	Zhang, Hong(zhangh@impcas.ac.cn)
英文摘要	The mitochondrial proteins involved in spermatogenic cells apoptosis in zebrafish after carbon ion radiation (CIR) were screened. The relative biological effectiveness (RBE) of CIR in zebrafish testes was investigated. Apoptosis of testicular cells was measured within 24hr following 1 and 4Gy CIR. Immunoblotting was used to assess the levels of mitochondrial apoptotic proteins in testes, and proliferative and apoptotic spermatogenic cells were detected by immunofluorescence after CIR. Label-free quantitative(LFQ) and parallel reaction monitoring-based target proteomics (PRM) were combined to screen and validate differential mitochondrial proteins in testes between 4Gy and control groups at 24hr after CIR. The RBE of CIR in zebrafish testes was 1.48 +/- 0.04, and induction of apoptosis by CIR was higher than that of X-rays in testicular cells. Mitochondrial apoptotic pathways play a crucial role in spermatogenic cells apoptosis after CIR, with 60 differential mitochondrial proteins identified. Among 20 target proteins, 12 were significantly upregulated, 2 were significantly downregulated in the 4Gy CIR group. The results of PRM were consistent with label-free analysis. This is the first study to screen the differential mitochondrial proteins and provide useful information to understand the underlying mechanisms of spermatogenic cell apoptosis in zebrafish following CIR.
资助项目	Ministry of Science and Technology National Key RD project[2016YFC0904600] ; Ministry of Science and Technology National Key RD project[SQ2018YFE020524] ; Key Program of National Natural Science Foundation of China[U1432248] ; National Natural Science Foundation of China[11505244] ; National Natural Science Foundation of China[11875061] ; Western Talent Program of the Chinese Academy of Sciences[Y560020XB0]
WOS关键词	LOW-DOSE RADIATION ; MOUSE TESTES ; IRRADIATION ; DEATH ; MODULATION ; AUTOPHAGY ; BEAMS
WOS研究方向	Cell Biology ; Physiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000484376600085
资助机构	Ministry of Science and Technology National Key RD project ; Key Program of National Natural Science Foundation of China ; National Natural Science Foundation of China ; Western Talent Program of the Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/141767]
专题	中国科学院近代物理研究所
通讯作者	Zhang, Hong
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Dept Radiat Med, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Gansu, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Key Lab Basic Res Heavy Ion Radiat Applicat Med, Lanzhou, Gansu, Peoples R China 4.Univ Chinese Acad Sci, Sch Nucl Sci & Technol, Beijing, Peoples R China 5.Gansu Wuwei Tumor Hosp, Dept Ultrasonog, Wuwei, Peoples R China 6.Gansu Wuwei Tumor Hosp, Dept Sci & Technol, Wuwei, Peoples R China
推荐引用方式 GB/T 7714	Li, Hongyan,Zhang, Weihong,Zhang, Hong,et al. Mitochondrial proteomics reveals the mechanism of spermatogenic cells apoptosis induced by carbon ion radiation in zebrafish[J]. JOURNAL OF CELLULAR PHYSIOLOGY,2019,234(12):22439-22449.
APA	Li, Hongyan.,Zhang, Weihong.,Zhang, Hong.,Xie, Yi.,Sun, Chao.,...&Yan, Junfang.(2019).Mitochondrial proteomics reveals the mechanism of spermatogenic cells apoptosis induced by carbon ion radiation in zebrafish.JOURNAL OF CELLULAR PHYSIOLOGY,234(12),22439-22449.
MLA	Li, Hongyan,et al."Mitochondrial proteomics reveals the mechanism of spermatogenic cells apoptosis induced by carbon ion radiation in zebrafish".JOURNAL OF CELLULAR PHYSIOLOGY 234.12(2019):22439-22449.