Inhibition of Wnt signalling pathway by XAV939 enhances radiosensitivity in human cervical cancer HeLa cells

doi:10.1080/21691401.2020.1716779

	Inhibition of Wnt signalling pathway by XAV939 enhances radiosensitivity in human cervical cancer HeLa cells
	Zhang, Jinhua 1,2,3; Si, Jing 1,2,3; Gan, Lu 1,2,3; Guo, Menghuan 4; Yan, Junfang 1,2,3; Chen, Yuhong 1,2,3; Wang, Fang 1,2,3; Xie, Yi 1,2,3; Wang, Yupei 1,2,3; Zhang, Hong 1,2,3
刊名	ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
	2020
卷号	48 期号:1 页码:479-487
关键词	C-12(6+) irradiation cervical carcinoma Wnt beta-catenin pathway XAV939 radio-sensitivity
ISSN号	2169-1401
DOI	10.1080/21691401.2020.1716779
通讯作者	Si, Jing(sijing@impcas.ac.cn) ; Zhang, Hong(zhangh@impcas.ac.cn)
英文摘要	Cervical cancer is the second most common malignant tumour threatening women's health. In recent years, heavy-ion beam therapy is becoming a newly emerging therapeutic mean of cancer; however, radio-resistance and radiation-induced damage constitute the main obstacles for curative treatment of cervical cancer. Therefore, to identify the radiosensitizers is essential. Here, we investigated the effects of Wnt signalling pathway on the response of C-12(6+) radiation in HeLa cells. XAV939, an inhibitor of Wnt signalling pathway, was added two hours before C-12(6+) radiation.C-12(6+) radiation inhibited the viability of HeLa cells in a time-dependent manner, and inhibiting Wnt signalling using XAV939 significantly intensified this stress. Meanwhile, C-12(6+) radiation induced a significant increased cell apoptosis, G2/M phase arrest, and the number of gamma-H2AX foci. Supplementation with XAV939 significantly increased the effects induced by C-12(6+) radiation alone. Combining XAV939 with C-12(6+) irradiation, the expression of apoptotic genes (p53, Bax, Bcl-2) was significantly increased, while the expression of Wnt-related genes (Wnt3a, Wnt5a, beta-catenin, cyclin D1 and c-Myc) was significantly decreased. Overall, these findings suggested that blockage of the Wnt/beta-catenin pathway effectively sensitizes HeLa cells to C-12(6+) irradiation, and it may be a potential therapeutic approach in terms of increasing the clinical efficacy of C-12(6+) beams.
资助项目	Ministry of Science and Technology National Key RD Project[2018YFE0205100] ; Key Program of the National Natural Science Foundation of China[U1632270] ; National Natural Science Foundation of China[11675234] ; National Natural Science Foundation of China[11605255] ; National Natural Science Foundation of China[11875061] ; Science and Technology Plan Project of Chengguan district, Lanzhou[2019RCCX0071] ; Lanzhou Talent Innovation and Entrepreneurship Project[2019-RC76]
WOS关键词	CARBON-ION RADIOTHERAPY ; WNT/BETA-CATENIN PATHWAY ; DIFFERENTIATION ; IRRADIATION ; ACTIVATION ; CARCINOMA ; RADIATION ; AUTOPHAGY ; IMPACT
WOS研究方向	Biotechnology & Applied Microbiology ; Engineering ; Materials Science
语种	英语
出版者	TAYLOR & FRANCIS LTD
WOS记录号	WOS:000509033500001
资助机构	Ministry of Science and Technology National Key RD Project ; Key Program of the National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science and Technology Plan Project of Chengguan district, Lanzhou ; Lanzhou Talent Innovation and Entrepreneurship Project
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/141455]
专题	中国科学院近代物理研究所
通讯作者	Si, Jing; Zhang, Hong
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Dept Radiat Med, Lanzhou, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Peoples R China 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 4.Lanzhou Univ, Sch Pharm, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jinhua,Si, Jing,Gan, Lu,et al. Inhibition of Wnt signalling pathway by XAV939 enhances radiosensitivity in human cervical cancer HeLa cells[J]. ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY,2020,48(1):479-487.
APA	Zhang, Jinhua.,Si, Jing.,Gan, Lu.,Guo, Menghuan.,Yan, Junfang.,...&Zhang, Hong.(2020).Inhibition of Wnt signalling pathway by XAV939 enhances radiosensitivity in human cervical cancer HeLa cells.ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY,48(1),479-487.
MLA	Zhang, Jinhua,et al."Inhibition of Wnt signalling pathway by XAV939 enhances radiosensitivity in human cervical cancer HeLa cells".ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY 48.1(2020):479-487.