Phycocyanin Ameliorates Radiation-Induced Acute Intestinal Toxicity by Regulating the Effect of the Gut Microbiota on the TLR4/Myd88/NF-kappa B Pathway

doi:10.1002/jpen.1744

	Phycocyanin Ameliorates Radiation-Induced Acute Intestinal Toxicity by Regulating the Effect of the Gut Microbiota on the TLR4/Myd88/NF-kappa B Pathway
	Lu, Lina 1,2; Li, Wenjun 3,4; Sun, Chao 5; Kang, Shuhe 3; Li, Jia 3; Luo, Xingping 3; Su, Qiong 3; Liu, Bin 1,6; Qin, Song 2,4
刊名	JOURNAL OF PARENTERAL AND ENTERAL NUTRITION
	2019-11-25
页码	10
关键词	gut microbiota intestinal injury phycocyanin radiation
ISSN号	0148-6071
DOI	10.1002/jpen.1744
通讯作者	Liu, Bin(binliu0736@126.com)
英文摘要	Background Radiation-induced gastrointestinal syndrome, including nausea, diarrhea, and dehydration, contributes to morbidity and mortality after medical or industrial radiation exposure, which seriously affects patient quality of life after treatment. No safe and effective radiation countermeasure has been approved for clinical therapy. In this study, we aimed to investigate the potential protective effects of phycocyanin (PC) against radiation-induced acute intestinal injury. Materials and Methods C57BL/6 mice were orally administered 50 mg/kg PC once per day for 1 month before exposure to total-abdominal x-ray irradiation at a single dose of 12 Gy. The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor kappa B (NF-kappa B) signaling were evaluated. Results Severe histopathological damage, such as intestinal mucosal epithelial cell apoptosis, necrosis, and nuclear rupture, was most clearly observed 24 hours after total-abdominal x-ray irradiation. Intestinal integrity was damaged by irradiation, which manifested in reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1). PC pretreatment significantly ameliorated radiation-induced intestinal injury. PC also modulated the gut microbiota composition, increasing the proportion of beneficial bacteria and decreasing that of harmful bacteria, which in turn lowered LPS levels and suppressed TLR4/Myd88/NF-kappa B pathway activation. Finally, levels of corresponding inflammatory cytokines, including tumor necrosis factor alpha and interleukin-6, were also downregulated. Conclusion PC protects against mouse intestinal injury from high-dose radiation by regulating the effect of the gut microbiota on the TLR4/Myd88/NF-kappa B pathway, suggesting PC as a promising natural radiation countermeasure.
资助项目	National Key Research and Development Program of China[2018YFD0901102] ; Key Research and Development Program of Yantai[Y933021011] ; National Natural Science Foundation of China[11775280] ; Northwest Minzu University's Double First-class and Characteristic Development Guide Special Funds-Chemistry Key Disciplines in Gansu Province[11080316] ; Fundamental Research Funds for the Central Universities[31920180020] ; Fundamental Research Funds for the Central Universities[31920190013] ; Fundamental Research Funds for the Central Universities[31920190018]
WOS关键词	INFLAMMATORY-BOWEL-DISEASE ; C-PHYCOCYANIN ; FECAL MICROBIOTA ; MOUSE ; PROTECTS ; INJURY
WOS研究方向	Nutrition & Dietetics
语种	英语
出版者	WILEY
WOS记录号	WOS:000498400500001
资助机构	National Key Research and Development Program of China ; Key Research and Development Program of Yantai ; National Natural Science Foundation of China ; Northwest Minzu University's Double First-class and Characteristic Development Guide Special Funds-Chemistry Key Disciplines in Gansu Province ; Fundamental Research Funds for the Central Universities
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/141218]
专题	中国科学院近代物理研究所
通讯作者	Liu, Bin
作者单位	1.Lanzhou Univ, Sch Nucl Sci & Technol, Lanzhou, Gansu, Peoples R China 2.Northwest Minzu Univ, Sch Chem Engn, Lanzhou, Gansu, Peoples R China 3.Chinese Acad Sci, Yantai Inst Costal Zone Res, Key Lab Biol & Bioresource Utilizat, Yantai, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Shandong, Peoples R China 5.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China 6.Lanzhou Univ, Sch Stomatol, Lanzhou, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Lu, Lina,Li, Wenjun,Sun, Chao,et al. Phycocyanin Ameliorates Radiation-Induced Acute Intestinal Toxicity by Regulating the Effect of the Gut Microbiota on the TLR4/Myd88/NF-kappa B Pathway[J]. JOURNAL OF PARENTERAL AND ENTERAL NUTRITION,2019:10.
APA	Lu, Lina.,Li, Wenjun.,Sun, Chao.,Kang, Shuhe.,Li, Jia.,...&Qin, Song.(2019).Phycocyanin Ameliorates Radiation-Induced Acute Intestinal Toxicity by Regulating the Effect of the Gut Microbiota on the TLR4/Myd88/NF-kappa B Pathway.JOURNAL OF PARENTERAL AND ENTERAL NUTRITION,10.
MLA	Lu, Lina,et al."Phycocyanin Ameliorates Radiation-Induced Acute Intestinal Toxicity by Regulating the Effect of the Gut Microbiota on the TLR4/Myd88/NF-kappa B Pathway".JOURNAL OF PARENTERAL AND ENTERAL NUTRITION (2019):10.