Suppression of innate immune signaling molecule, MAVS, reduces radiation-induced bystander effect

doi:10.1080/09553002.2020.1807642

	Suppression of innate immune signaling molecule, MAVS, reduces radiation-induced bystander effect
	Jia, Rong 1,2,3; Chen, Yaxiong 1,2; Jia, Cong 4; Hu, Burong 1,2,5; Du, Yarong 1,2,4
刊名	INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
	2020-08-19
页码	9
关键词	MAVS irradiation ROS RIBE
ISSN号	0955-3002
DOI	10.1080/09553002.2020.1807642
通讯作者	Du, Yarong(duyrlive@impcas.ac.cn)
英文摘要	Purpose Mitochondrial antiviral signaling (MAVS) protein, located in the mitochondrial out-membrane, is necessary for IFN-beta induction and IFN-stimulated gene expression in response to external stress such as viral invasion and ionizing radiation (IR). Although the involvement of radiation induced bystander effect (RIBE) has been investigated for decades for secondary cancer risk related to radiotherapy, the underlying regulatory mechanisms remain largely unclear, especially the roles played by the immune factors such as MAVS. Material and methods MAVS gene knockout cells using CRISPR/Cas9 technology were used as donor cells or recipient cells to assess the role of MAVS in RIBE by means of co-cultured system. The micronucleus and gamma H2AX foci in the recipient cells were counted to demonstrate the degree of RIBE. The reactive oxygen species (ROS) level in the recipient was measured using the fluorescent dye 2 ' 7 '-dichlorofluorescein. Results Firstly, we found that MAVS expression level was different in A549, BEAS-2B, U937 and HepG2 cells. Cell co-culture experiments showed that MAVS participate in RIBE. Interestingly, the RIBE response was more significant in recipient cells with higher level of MAVS (i.e. A549) than that in recipient cells showing lower level of MAVS (i.e. BEAS-2B). Further, the bystander response was dramatically suppressed in MAVS-silenced A549 and BEAS-2B recipient cells. MAVS-silenced recipient cells exhibited lower level of ROS induced by IR. Conclusions Our results indicated that the innate immune signaling molecule MAVS in recipient cells participate in RIBE. ROS is an important factor in RIBE via MAVS pathway and MAVS may be a potential target for the precise radiotherapy and radioprotection.
资助项目	National Natural Science Foundation of China[11705248] ; National Natural Science Foundation of China[U1832101] ; National Natural Science Foundation of China[11635013] ; Science and Technology Research Project of Gansu Province[145RTSA012] ; Science and Technology Research Project of Gansu Province[17JR5RA307]
WOS关键词	OXIDATIVE STRESS ; DYNAMICS
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Nuclear Science & Technology ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	TAYLOR & FRANCIS LTD
WOS记录号	WOS:000561846400001
资助机构	National Natural Science Foundation of China ; Science and Technology Research Project of Gansu Province
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/139869]
专题	中国科学院近代物理研究所
通讯作者	Du, Yarong
作者单位	1.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Peoples R China 2.Chinese Acad Sci, Key Lab Space Radiobiol Gansu Prov, Inst Modern Phys, Lanzhou, Peoples R China 3.Univ Chinese Acad Sci, Inst Modern Phys, Beijing, Peoples R China 4.Northwest Normal Univ, Coll Life Sci, Lanzhou, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Jia, Rong,Chen, Yaxiong,Jia, Cong,et al. Suppression of innate immune signaling molecule, MAVS, reduces radiation-induced bystander effect[J]. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY,2020:9.
APA	Jia, Rong,Chen, Yaxiong,Jia, Cong,Hu, Burong,&Du, Yarong.(2020).Suppression of innate immune signaling molecule, MAVS, reduces radiation-induced bystander effect.INTERNATIONAL JOURNAL OF RADIATION BIOLOGY,9.
MLA	Jia, Rong,et al."Suppression of innate immune signaling molecule, MAVS, reduces radiation-induced bystander effect".INTERNATIONAL JOURNAL OF RADIATION BIOLOGY (2020):9.