Fluorescent Probes for Imaging Protein Disulfides in Live Organisms

doi:10.1021/acssensors.1c00049

	Fluorescent Probes for Imaging Protein Disulfides in Live Organisms
	Hu, Guodong 1,3; Zhong, Miao 1,3; Zhao, Jintao 1,3; Gao, Hao 4; Gan, Lu 2; Zhang, Hong 2; Zhang, Shengxiang 4; Fang, Jianguo 1,3
刊名	ACS SENSORS
	2021-03-26
卷号	6 期号:3 页码:1384-1391
关键词	fluorescent probes environment-sensitive probes protein disulfides in vivo imaging two-photon mouse stroke model
ISSN号	2379-3694
DOI	10.1021/acssensors.1c00049
通讯作者	Fang, Jianguo(fangjg@lzu.edu.cn)
英文摘要	Cellular redox homeostasis is predominantly controlled by the ratio of thiols and disulfides, and reversible thioldisulfide exchange reactions are fundamental of the biological redox regulation. However, due to the dynamic exchanges of thiols and disulfides, the detection, especially the in situ detection, of protein disulfides (PDS) is challenging. We employ the strategy, i.e., the increase of emission upon an environment-sensitive dye binding to proteins, to design PDS probes and discover a two-photon probe PDSTP590 (S6) that selectively recognizes PDS in live organisms. With the aid of the probe, we further disclose the elevation of PDS in brains of the mouse stroke model.
资助项目	National Natural Science Foundation of China[21778028] ; National Natural Science Foundation of China[22077055] ; Natural Science Foundation of Gansu Province[20JR5RA311] ; Natural Science Foundation of Gansu Province[18JR4RA003] ; 111 project
WOS研究方向	Chemistry ; Science & Technology - Other Topics
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000635484500086
资助机构	National Natural Science Foundation of China ; Natural Science Foundation of Gansu Province ; 111 project
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/137604]
专题	中国科学院近代物理研究所
通讯作者	Fang, Jianguo
作者单位	1.Lanzhou Univ, Coll Chem & Chem Engn, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Dept Heavy Ion Radiat Med, Lanzhou 730000, Peoples R China 3.Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China 4.Lanzhou Univ, Sch Life Sci, Gansu Key Lab Biomonitoring & Bioremediat Environ, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Hu, Guodong,Zhong, Miao,Zhao, Jintao,et al. Fluorescent Probes for Imaging Protein Disulfides in Live Organisms[J]. ACS SENSORS,2021,6(3):1384-1391.
APA	Hu, Guodong.,Zhong, Miao.,Zhao, Jintao.,Gao, Hao.,Gan, Lu.,...&Fang, Jianguo.(2021).Fluorescent Probes for Imaging Protein Disulfides in Live Organisms.ACS SENSORS,6(3),1384-1391.
MLA	Hu, Guodong,et al."Fluorescent Probes for Imaging Protein Disulfides in Live Organisms".ACS SENSORS 6.3(2021):1384-1391.