Mechanism of Fuzheng Kang'ai Formula Regulating Tumor Microenvironment in Non-Small Cell Lung Cancer

doi:10.1007/s11655-021-3451-1

	Mechanism of Fuzheng Kang'ai Formula Regulating Tumor Microenvironment in Non-Small Cell Lung Cancer
	Tian Yun-ling 4; Fu Song-bo 4; Li Bo 4; Yuan Ling-yan 4; Bing Zhi-tong 1,2,3
刊名	CHINESE JOURNAL OF INTEGRATIVE MEDICINE
	2021-09-21
页码	9
关键词	network pharmacology prognosis tumor microenvironment Fuzheng Kang'ai Formula Chinese medicine lung adenocarcinoma
ISSN号	1672-0415
DOI	10.1007/s11655-021-3451-1
通讯作者	Tian Yun-ling(yunlingtian7318@126.com)
英文摘要	Objective: To study the mechanism of Chinese herbal medicine Fuzheng Kang'ai Formula (FZKA) on tumor microenvironment (TME). Methods: CIBERSORTx was used for analysis of TME. Traditional Chinese Medicine Systems Pharmacology and Analysis Platform was applied to identify compounds-targets network and the Cancer Genome Atlas (TCGA) was employed to identify the differential expression genes (DEGs) between tumor and paracancerous tissues in lung adenocarcinoma (LUAD) from TCGA-LUAD. Additionally, DEGs with prognosis in LUAD was calculated by univariable and multivariate Cox regression. The core targets of FZKA were analyzed in lung adenocarcinoma TME. Protein-protein interaction database was employed to predict down-stream of target. Quantitative reverse transcription polymerase chain reaction was employed for biological experiment in A549, H1299 and PC9 cell lines. Results: The active and resting mast cells were significantly associated with prognosis of LUAD (P<0.05). Of the targets, CCNA2 as an important target of FZKA (hazard ratio=1.41, 95% confidential interval: 1.01-2.01, P<0.05) was a prognostic target and significantly associated with mast cells. CCNA2 was positively correlated with mast cell activation and negatively correlated with mast cell resting state. BCL1L2, ACTL6A and ITGAV were down-stream of CCNA2, which were validated by qRT-PCR in A549 cell. Conclusion: FZKA could directly bind to CCNA2 and inhibit tumor growth by regulating CCNA2 downstream genes and TME of NSCLC closely related to CCNA2.
WOS关键词	CHINESE HERBAL MEDICINE ; ALTERNATIVE MEDICINE ; COMPLEMENTARY ; NETWORK ; BLOCKADE ; THERAPY ; QUALITY
WOS研究方向	Integrative & Complementary Medicine
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000698067400004
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/136533]
专题	中国科学院近代物理研究所
通讯作者	Tian Yun-ling
作者单位	1.Univ Chinese Acad Sci, Sch Nucl Sci & Technol, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Dept Computat Phys, Lanzhou 730000, Peoples R China 3.Lanzhou Univ, Evidence Based Med Ctr, Sch Basic Med Sci, Lanzhou 730000, Peoples R China 4.Lanzhou Univ, Dept Endocrinol & Metab, Hosp 1, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Tian Yun-ling,Fu Song-bo,Li Bo,et al. Mechanism of Fuzheng Kang'ai Formula Regulating Tumor Microenvironment in Non-Small Cell Lung Cancer[J]. CHINESE JOURNAL OF INTEGRATIVE MEDICINE,2021:9.
APA	Tian Yun-ling,Fu Song-bo,Li Bo,Yuan Ling-yan,&Bing Zhi-tong.(2021).Mechanism of Fuzheng Kang'ai Formula Regulating Tumor Microenvironment in Non-Small Cell Lung Cancer.CHINESE JOURNAL OF INTEGRATIVE MEDICINE,9.
MLA	Tian Yun-ling,et al."Mechanism of Fuzheng Kang'ai Formula Regulating Tumor Microenvironment in Non-Small Cell Lung Cancer".CHINESE JOURNAL OF INTEGRATIVE MEDICINE (2021):9.