CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer

doi:10.1186/s12885-019-6017-2

	CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer
	Li, Hongbin 1,2,3,4; Jin, Xiaodong 1,2,3,4; Liu, Bingtao 1,2,3,4; Zhang, Pengcheng 1,2,3,4; Chen, Weiqiang 1,2,3,4; Li, Qiang 1,2,3,4
刊名	BMC CANCER
	2019-08-22
卷号	19 期号:1 页码:14
关键词	Colon cancer cells Circular RNA CBL 11 MiR-6778-5p YWHAE
ISSN号	1471-2407
DOI	10.1186/s12885-019-6017-2
其他题名	CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer
通讯作者	Li, Qiang(liqiang@impcas.ac.cn)
英文摘要	Background Radiotherapy (RT) is considered an important therapeutic strategy in the fight against colorectal cancer (CRC). However, the existence of some radioresistance factors becomes the main challenge for the RT. Recently, non-coding RNAs (ncRNAs) have shown an important role in modulating cancer cell responses to ionizing radiation (IR). It is therefore of great significance to elucidate the exact mechanisms of ncRNAs in IR-mediated responses to CRC. Methods Microarrays were used to identify specific miRNAs that may be altered in response to IR. Bioinformatics, luciferase reporter analyses were used to explore the targets of miR-6778-5p. CircRNA CBL.11 was identified to bind with miR-6778-5p by bioinformatic analysis, AGO2 immunoprecipitation and biotinylated RNA pull-down assay. Functional experiments, including CCK-8 assay, cell colony formation assay and EdU incorporation were conducted to investigate the biological roles of miR-6778-5p and circular RNA CBL.11. Results MiR-6778-5p was suppressed in CRC cells after irradiation. Results of functional experiments indicated that miR-6778-5p promoted the proliferation of CRC cells. Luciferase reporter analyses showed that YWHAE was a target of miR-6778-5p, which mediated the function of miR-6778-5p in the proliferation of CRC cells via the p53 pathway. Furthermore, we have noticed that after carbon ion irradiation, circRNA CBL.11 was increased in CRC cells and could function as a competing endogenous RNA (ceRNA) to regulate YWHAE expression by sponging miR-6778-5p, resulting in regulation the proliferation of CRC cells. Conclusion CircRNA CBL.11 may play an important role in improving the efficacy of carbon ion RT against CRC.
资助项目	National Key Research and Development Program of China[2018YFC0115700] ; National Key Research and Development Program of China[2018YFC0115702] ; National Natural Science Foundation of China[11875299] ; National Natural Science Foundation of China[U1532264]
WOS关键词	INDUCED DNA-DAMAGE ; UP-REGULATION ; NONCODING RNA ; RADIATION ; METASTASIS ; EXPRESSION ; CARCINOMA ; P53 ; RADIOSENSITIVITY ; RADIORESISTANCE
WOS研究方向	Oncology
语种	英语
出版者	BMC
WOS记录号	WOS:000483314400005
资助机构	National Key Research and Development Program of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/132772]
专题	中国科学院近代物理研究所
通讯作者	Li, Qiang
作者单位	1.Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Key Lab Basic Res Heavy Ion Radiat Applicat Med, Lanzhou 730000, Gansu, Peoples R China 4.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Li, Hongbin,Jin, Xiaodong,Liu, Bingtao,et al. CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer[J]. BMC CANCER,2019,19(1):14.
APA	Li, Hongbin,Jin, Xiaodong,Liu, Bingtao,Zhang, Pengcheng,Chen, Weiqiang,&Li, Qiang.(2019).CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer.BMC CANCER,19(1),14.
MLA	Li, Hongbin,et al."CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer".BMC CANCER 19.1(2019):14.