MORF9 increases the RNA-binding activity of PLS-type pentatricopeptide repeat protein in plastid RNA editing

doi:10.1600/036364417X696032

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院光生物学重点实验室

	MORF9 increases the RNA-binding activity of PLS-type pentatricopeptide repeat protein in plastid RNA editing
	Yan, Junjie 2; Zhang, Qunxia 2; Guan, Zeyuan 2; Wang, Qiang 2; Li, Li 2; Ruan, Fengying 2; Lin, Rongcheng 1; Zou, Tingting 2; Yin, Ping 2
刊名	NATURE PLANTS
	2017
卷号	3 期号:5
ISSN号	2055-026X
DOI	10.1600/036364417X696032
文献子类	Article
英文摘要	RNA editing is a post-transcriptional process that modifies the genetic information on RNA molecules. In flowering plants, RNA editing usually alters cytidine to uridine in plastids and mitochondria. The PLS-type pentatricopeptide repeat (PPR) protein and the multiple organellar RNA editing factor (MORF, also known as RNA editing factor interacting protein (RIP)) are two types of key trans-acting factors involved in this process. However, how they cooperate with one another remains unclear. Here, we have characterized the interactions between a designer PLS-type PPR protein (PLS)(3)PPR and MORF9, and found that RNA-binding activity of (PLS)(3)PPR is drastically increased on MORF9 binding. We also determined the crystal structures of (PLS)(3)PPR, MORF9 and the (PLS)(3)PPR-MORF9 complex. MORF9 binding induces significant compressed conformational changes of (PLS)(3)PPR, revealing the molecular mechanisms by which MORF9-bound (PLS)(3)PPR has increased RNA-binding activity. Similarly, increased RNA-binding activity is observed for the natural PLS-type PPR protein, LPA66, in the presence of MORF9. These findings significantly expand our understanding of MORF function in plant organellar RNA editing.
学科主题	Plant Sciences ; Evolutionary Biology
电子版国际标准刊号	2055-0278
出版地	LONDON
WOS关键词	SINGLE-STRANDED RNA ; STRUCTURAL BASIS ; ARABIDOPSIS-THALIANA ; DYW DOMAIN ; RECOGNITION ; PLANTS ; MAIZE ; MITOCHONDRIA ; BIOGENESIS ; SOFTWARE
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000409055400006
资助机构	Ministry of Science and Technology [2015CB910900] ; Fok Ying-Tong Education FoundationFok Ying Tung Education Foundation [151021] ; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [91535301] ; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [2014PY026, 2015PY219, 2014JQ001] ; Huazhong Agricultural University Scientific & Technological Self-innovation Foundation [2013RC013] ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2015M572163]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22362]
专题	中科院光生物学重点实验室
作者单位	1.Huazhong Agr Univ, Natl Ctr Plant Gene Res, Wuhan 430070, Peoples R China 2.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Yan, Junjie,Zhang, Qunxia,Guan, Zeyuan,et al. MORF9 increases the RNA-binding activity of PLS-type pentatricopeptide repeat protein in plastid RNA editing[J]. NATURE PLANTS,2017,3(5).
APA	Yan, Junjie.,Zhang, Qunxia.,Guan, Zeyuan.,Wang, Qiang.,Li, Li.,...&Yin, Ping.(2017).MORF9 increases the RNA-binding activity of PLS-type pentatricopeptide repeat protein in plastid RNA editing.NATURE PLANTS,3(5).
MLA	Yan, Junjie,et al."MORF9 increases the RNA-binding activity of PLS-type pentatricopeptide repeat protein in plastid RNA editing".NATURE PLANTS 3.5(2017).