Biodegradation Mechanisms of Patulin in Candida guilliermondii: An iTRAQ-Based Proteomic Analysis

doi:10.1093/hmg/ddx147

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	Biodegradation Mechanisms of Patulin in Candida guilliermondii: An iTRAQ-Based Proteomic Analysis
	Chen, Yong ; Peng, Huai-Min ; Wang, Xiao ; Li, Bo-Qiang ; Long, Man-Yuan ; Tian, Shi-Ping
刊名	TOXINS
	2017
卷号	9 期号:2
关键词	contamination detoxification mycotoxins patulin Penicillium yeast
ISSN号	2072-6651
DOI	10.1093/hmg/ddx147
文献子类	Article
英文摘要	Patulin, a potent mycotoxin, contaminates fruits and derived products worldwide, and is a serious health concern. Several yeast strains have shown the ability to effectively degrade patulin. However, the mechanisms of its biodegradation still remain unclear at this time. In the present study, biodegradation and involved mechanisms of patulin by an antagonistic yeast Candida guilliermondii were investigated. The results indicated that C. guilliermondii was capable of not only multiplying to a high population in medium containing patulin, but also effectively reducing patulin content in culture medium. Degradation of patulin by C. guilliermondii was dependent on the yeast cell viability, and mainly occurred inside cells. E-ascladiol was the main degradation product of patulin. An iTRAQ-based proteomic analysis revealed that the responses of C. guilliermondii to patulin were complex. A total of 30 differential proteins involved in 10 biological processes were identified, and more than two-thirds of the differential proteins were down-accumulated. Notably, a short-chain dehydrogenase (gi\| 190348612) was markedly induced by patulin at both the protein and mRNA levels. Our findings will provide a foundation to help enable the commercial development of an enzyme formulation for the detoxification of patulin in fruit-derived products.
学科主题	Biochemistry & Molecular Biology ; Genetics & Heredity
出版地	BASEL
WOS关键词	BIOLOGICAL-CONTROL ; PENICILLIUM-EXPANSUM ; MYCOTOXIN PATULIN ; GENE-EXPRESSION ; BIOCONTROL ; DETOXIFICATION ; BIOSYNTHESIS ; DEGRADATION ; INSIGHTS ; REVEALS
语种	英语
出版者	MDPI
WOS记录号	WOS:000405102100005
资助机构	National Key Research and Development Program of China [2016YFD0400902] ; National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31371863, 31530057] ; Youth Innovation Promotion Association CAS [2015063]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22357]
专题	中科院北方资源植物重点实验室
作者单位	1.Univ Chicago, Dept Ecol & Evolut, 940 E 57Th St, Chicago, IL 60637 USA 2.Li, Bo-Qiang 3.Wang, Xiao 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Peng, Huai-Min 7.[Chen, Yong
推荐引用方式 GB/T 7714	Chen, Yong,Peng, Huai-Min,Wang, Xiao,et al. Biodegradation Mechanisms of Patulin in Candida guilliermondii: An iTRAQ-Based Proteomic Analysis[J]. TOXINS,2017,9(2).
APA	Chen, Yong,Peng, Huai-Min,Wang, Xiao,Li, Bo-Qiang,Long, Man-Yuan,&Tian, Shi-Ping.(2017).Biodegradation Mechanisms of Patulin in Candida guilliermondii: An iTRAQ-Based Proteomic Analysis.TOXINS,9(2).
MLA	Chen, Yong,et al."Biodegradation Mechanisms of Patulin in Candida guilliermondii: An iTRAQ-Based Proteomic Analysis".TOXINS 9.2(2017).