Identification and Functional Characterization of a Tonoplast Dicarboxylate Transporter in Tomato (Solanum lycopersicum)

doi:10.11646/phytotaxa.316.2.2

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	Identification and Functional Characterization of a Tonoplast Dicarboxylate Transporter in Tomato (Solanum lycopersicum)
	Liu, Ruiling 1; Li, Boqiang ; Qin, Guozheng ; Zhang, Zhanquan ; Tian, Shiping 1
刊名	FRONTIERS IN PLANT SCIENCE
	2017
卷号	8
关键词	tomato tonoplast transporters overexpression RNAi organic acid composition
ISSN号	1664-462X
DOI	10.11646/phytotaxa.316.2.2
文献子类	Article
英文摘要	Acidity plays an important role in flavor and overall organoleptic quality of fruit and is mainly due to the presence of organic acids. Understanding the molecular basis of organic acid metabolism is thus of primary importance for fruit quality improvement. Here, we cloned a putative tonoplast dicarboxylate transporter gene (SlTDT) from tomato, and submitted it to the NCBI database (GenBank accession number: KC733165). SlTDT protein contained 13 putative transmembrane domains in silico analysis. Confocal microscopic study using green fluorescent fusion proteins revealed that SlTDT was localized on tonoplast. The expression patterns of SlTDT in tomato were analyzed by RT-qPCR. The results indicated that SlTDT expressed in leaves, roots, flowers and fruits at different ripening stages, suggesting SlTDT may be associated with the development of different tissues. To further explore the function of SlTDT, we constructed both overexpression and RNAi vectors and obtained transgenic tomato plants by agrobacterium-mediated method. Gas chromatographymass spectrometer (GC-MS) analysis showed that overexpression of SlTDT significantly increased malate content, and reduced citrate content in tomato fruit. By contrast, repression of SlTDT in tomato reduced malate content of and increased citrate content. These results indicated that SlTDT played an important role in remobilization of malate and citrate in fruit vacuoles.
学科主题	Plant Sciences
出版地	LAUSANNE
WOS关键词	FRUIT-DEVELOPMENT ; APPLE FRUIT ; ORGANIC-ACIDS ; MALATE ; METABOLISM ; EXPRESSION ; ACCUMULATION ; CHANNEL ; MEMBRANE ; BERRIES
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000406967500002
资助机构	National Key Research and Development Program of China [2016YFD0400902] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530057] ; Youth Innovation Promotion Association CAS [2015063]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22355]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Ruiling,Li, Boqiang,Qin, Guozheng,et al. Identification and Functional Characterization of a Tonoplast Dicarboxylate Transporter in Tomato (Solanum lycopersicum)[J]. FRONTIERS IN PLANT SCIENCE,2017,8.
APA	Liu, Ruiling,Li, Boqiang,Qin, Guozheng,Zhang, Zhanquan,&Tian, Shiping.(2017).Identification and Functional Characterization of a Tonoplast Dicarboxylate Transporter in Tomato (Solanum lycopersicum).FRONTIERS IN PLANT SCIENCE,8.
MLA	Liu, Ruiling,et al."Identification and Functional Characterization of a Tonoplast Dicarboxylate Transporter in Tomato (Solanum lycopersicum)".FRONTIERS IN PLANT SCIENCE 8(2017).