A Genetic Transformation Method for Cadmium Hyperaccumulator Sedum plumbizincicola and Non-hyperaccumulating Ecotype of Sedum alfredii

doi:10.1086/692764

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	A Genetic Transformation Method for Cadmium Hyperaccumulator Sedum plumbizincicola and Non-hyperaccumulating Ecotype of Sedum alfredii
	Liu, Huan 3; Zhao, Haixia 3; Wu, Longhua 2; Xu, Wenzhong
刊名	FRONTIERS IN PLANT SCIENCE
	2017
卷号	8
关键词	Agrobacterium tumefaciens genetic transformation cadmium hyperaccumulator multiple shoot buds Sedum alfredii Sedum plumbizincicola
ISSN号	1664-462X
DOI	10.1086/692764
文献子类	Article
英文摘要	The present study demonstrates the development of an Agrobacterium-mediated genetic transformation method for species of the Sedum genus, which includes the Cd/Zn hyperaccumulator Sedum plumbizincicola and the non-hyperaccumulating ecotype of S. alfredii. Multiple shoots were induced from stem nodes of two Sedum plants using Murashige and Skoog (MS) medium containing 0.1 mg/L cytokinin 6-benzyladenine (6-BA) and 1.0 mg/L auxin 1-naphthaleneacetic acid (NAA). The shoot primordia were used as direct targets for Agrobacterium infection. Selection on hygromycin was highly effective in generating Agrobacterium-transformed explants. This callus-free procedure allowed us to obtain transgenic plantlets after rooting hygromycin-resistant shoots on phytohormone-free MS medium containing the antibiotic. The presence and expression of the reporter genes gusA and GFP in transgenic plants were confirmed by a real-time polymerase chain reaction, histochemical GUS assays, and confocal microscopy. This reliable method for genetic transformation of Sedum plants will help us to understand gene functions and the molecular mechanisms underlying Cd hypertolerance and hyperaccumulation in these species.
学科主题	Plant Sciences
出版地	LAUSANNE
WOS关键词	AGROBACTERIUM-MEDIATED TRANSFORMATION ; CRASSULACEAN ACID METABOLISM ; METAL HYPERACCUMULATION ; MOLECULAR-MECHANISMS ; LEAF EXPLANTS ; CD TOLERANCE ; KEY ROLE ; REGENERATION ; PLANTS ; L.
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000407712400005
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41325003, 91117010] ; Knowledge Innovation Programs of CAS [KSCX2-YW-G053]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22349]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing, Jiangsu, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Huan,Zhao, Haixia,Wu, Longhua,et al. A Genetic Transformation Method for Cadmium Hyperaccumulator Sedum plumbizincicola and Non-hyperaccumulating Ecotype of Sedum alfredii[J]. FRONTIERS IN PLANT SCIENCE,2017,8.
APA	Liu, Huan,Zhao, Haixia,Wu, Longhua,&Xu, Wenzhong.(2017).A Genetic Transformation Method for Cadmium Hyperaccumulator Sedum plumbizincicola and Non-hyperaccumulating Ecotype of Sedum alfredii.FRONTIERS IN PLANT SCIENCE,8.
MLA	Liu, Huan,et al."A Genetic Transformation Method for Cadmium Hyperaccumulator Sedum plumbizincicola and Non-hyperaccumulating Ecotype of Sedum alfredii".FRONTIERS IN PLANT SCIENCE 8(2017).