A Light Harvesting Complex- Like Protein in Maintenance of Photosynthetic Components in Chlamydomonas

doi:10.3897/phytokeys.79.11215

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	A Light Harvesting Complex- Like Protein in Maintenance of Photosynthetic Components in Chlamydomonas
	Zhao, Lei ; Cheng, Dongmei 2; Huang, Xiahe 3; Chen, Mei ; Dall'Osto, Luca ; Xing, Jiale 2; Gao, Liyan 3; Li, Lingyu 2; Wang, Yale 2; Bassi, Roberto 4
刊名	PLANT PHYSIOLOGY
	2017
卷号	174 期号:4 页码:2419-2433
ISSN号	0032-0889
DOI	10.3897/phytokeys.79.11215
文献子类	Article
英文摘要	Using a genetic approach, we have identified and characterized a novel protein, named Msf1 (Maintenance factor for photosystem I), that is required for the maintenance of specific components of the photosynthetic apparatus in the green alga Chlamydomonas reinhardtii. Msf1 belongs to the superfamily of light-harvesting complex proteins with three transmembrane domains and consensus chlorophyll-binding sites. Loss of Msf1 leads to reduced accumulation of photosystem I and chlorophyll-binding proteins/complexes. Msf1is a component of a thylakoid complex containing key enzymes of the tetrapyrrole biosynthetic pathway, thus revealing a possible link between Msf1 and chlorophyll biosynthesis. Protein interaction assays and greening experiments demonstrate that Msf1 interacts with Copper target homolog1 (CHL27B) and accumulates concomitantly with chlorophyll in Chlamydomonas, implying that chlorophyll stabilizes Msf1. Contrary to other light-harvesting complex-like genes, the expression of Msf1 is not stimulated by high-light stress, but its protein level increases significantly under heat shock, iron and copper limitation, as well as in stationary cells. Based on these results, we propose that Msf1 is required for the maintenance of photosystem I and specific protein-chlorophyll complexes especially under certain stress conditions.
学科主题	Plant Sciences
电子版国际标准刊号	1532-2548
出版地	ROCKVILLE
WOS关键词	CAB-LIKE PROTEINS ; SYNECHOCYSTIS-SP PCC-6803 ; SP PCC 6803 ; PSI-LHCI SUPERCOMPLEX ; A/B-BINDING-PROTEINS ; PLANT PHOTOSYSTEM-I ; THYLAKOID MEMBRANES ; STRESS-PROTEINS ; CHLOROPHYLL SYNTHESIS ; REINHARDTII
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000399812300001
资助机构	Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2015CB150100] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270288, 31470340, 31400212] ; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB17030100] ; Foreign Expert Program of the Chinese Academy of Sciences ; Marie Curie Actions Initial Training Networks ACCLIPHOT [PITN-GA-2012-316427]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22094]
专题	中科院光生物学重点实验室
作者单位	1.Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 200234, Peoples R China 2.Chinese Acad Sci, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, State Key Lab Mol Dev Biol, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 5.Univ Verona, Dipartimento Biotechnol, I-37134 Verona, Italy 6.Univ Geneva, Dept Plant Biol, CH-1211 Geneva, Switzerland 7.Univ Geneva, Dept Mol Biol, CH-1211 Geneva, Switzerland
推荐引用方式 GB/T 7714	Zhao, Lei,Cheng, Dongmei,Huang, Xiahe,et al. A Light Harvesting Complex- Like Protein in Maintenance of Photosynthetic Components in Chlamydomonas[J]. PLANT PHYSIOLOGY,2017,174(4):2419-2433.
APA	Zhao, Lei.,Cheng, Dongmei.,Huang, Xiahe.,Chen, Mei.,Dall'Osto, Luca.,...&Huang, Fang.(2017).A Light Harvesting Complex- Like Protein in Maintenance of Photosynthetic Components in Chlamydomonas.PLANT PHYSIOLOGY,174(4),2419-2433.
MLA	Zhao, Lei,et al."A Light Harvesting Complex- Like Protein in Maintenance of Photosynthetic Components in Chlamydomonas".PLANT PHYSIOLOGY 174.4(2017):2419-2433.