Establishment of a gene function analysis system for the euhalophyte Salicornia europaea L.

doi:10.1002/2017JG003991

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院植物分子生理学重点实验室

	Establishment of a gene function analysis system for the euhalophyte Salicornia europaea L.
	Tai, Fang 1; Lv, Sulian ; Jiang, Ping ; Wang, Jinhui 1; Feng, Juanjuan ; Li, Yinxin
刊名	PLANT CELL REPORTS
	2017
卷号	36 期号:8 页码:1251-1261
关键词	Salicornia europaea L. Suspension cells In vitro Agrobacterium-mediated transformation SeNHX1
ISSN号	0721-7714
DOI	10.1002/2017JG003991
文献子类	Article
英文摘要	The exploration of salt-tolerant genes from halophyte has seriously been limited by the lack of self-dependent transformation system. Here, an Agrobacterium tumefaciens-mediated in vitro cell transformation system of euhalophyte Salicornia europaea L. was developed. Calli derived from hypocotyl of S. europaea were co-cultured for 3 days with Agrobacterium at OD600 ranging from 1.0 to 1.5 and then selected with 25 mg/L hygromycin (Hyg). The transformed cells were identified from Hyg positive calli by GUS assay and qRT-PCR, and the transformation efficiency was up to 74.4%. The practicality of this system was further tested via genetic manipulation of S. europaea Na+/H+ antiporter 1 (SeNHX1) gene by creating the overexpressing, silencing, and empty vector cells. Survival ratio and Na+ distribution under salt treatment showed obvious differences in SeNHX1-overexpressing, -silencing, and empty vector cells, indicating the feasibility of this system to analyze gene function. This investigation is enlightening for studies in other non-model plants lacking of self-dependent transformation system.
学科主题	Environmental Sciences & Ecology ; Geology
电子版国际标准刊号	1432-203X
出版地	NEW YORK
WOS关键词	COMPARATIVE PROTEOMIC ANALYSIS ; IN-VITRO PLANTLET ; SALT TOLERANCE ; MESEMBRYANTHEMUM-CRYSTALLINUM ; KOSTELETZKYA-VIRGINICA ; CALLUS ; TOBACCO ; CULTURE ; NACL ; TRANSFORMATION
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000414961500002
资助机构	National Research and Development Project of Transgenic Crops of China [2016ZX08009-003-002] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270421]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22002]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Plant Mol Physiol, Inst Bot, 20 Nanxincun, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Tai, Fang,Lv, Sulian,Jiang, Ping,et al. Establishment of a gene function analysis system for the euhalophyte Salicornia europaea L.[J]. PLANT CELL REPORTS,2017,36(8):1251-1261.
APA	Tai, Fang,Lv, Sulian,Jiang, Ping,Wang, Jinhui,Feng, Juanjuan,&Li, Yinxin.(2017).Establishment of a gene function analysis system for the euhalophyte Salicornia europaea L..PLANT CELL REPORTS,36(8),1251-1261.
MLA	Tai, Fang,et al."Establishment of a gene function analysis system for the euhalophyte Salicornia europaea L.".PLANT CELL REPORTS 36.8(2017):1251-1261.