The transcription factor VaNAC17 from grapevine (Vitis amurensis) enhances drought tolerance by modulating jasmonic acid biosynthesis in transgenic Arabidopsis

doi:10.1007/s00299-020-02519-x

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	The transcription factor VaNAC17 from grapevine (Vitis amurensis) enhances drought tolerance by modulating jasmonic acid biosynthesis in transgenic Arabidopsis
	Su, Lingye 1,2,3; Fang, Linchuan 1; Zhu, Zhenfei 4; Zhang, Langlang 1; Sun, Xiaoming 1; Wang, Yi 2,4; Wang, Qingfeng 1; Li, Shaohua 2; Xin, Haiping 1
刊名	PLANT CELL REPORTS
	2020
卷号	39 期号:5 页码:621-634
关键词	Drought stress Jasmonic acid VaNAC17 Vitis amurensis
ISSN号	0721-7714
DOI	10.1007/s00299-020-02519-x
文献子类	Article
英文摘要	Key message Expression of VaNAC17 improved drought tolerance in transgenic Arabidopsis by upregulating stress-responsive genes, modulating JA biosynthesis, and enhancing ROS scavenging. Water deficit severely affects the growth and development of plants such as grapevine (Vitis spp.). Members of the NAC (NAM, ATAF1/2, and CUC2) transcription factor (TF) family participate in drought-stress-induced signal transduction in plants, but little is known about the roles of NAC genes in drought tolerance in grapevine. Here, we explored the role of VaNAC17 in Vitis amurensis, a cold-hardy, drought-tolerant species of grapevine. VaNAC17 was strongly induced in grapevine by drought, exogenous abscisic acid (ABA), and methyl jasmonate (MeJA). A transient expression assay in yeast indicated that VaNAC17 functions as a transcriptional activator. Notably, heterologous expression of VaNAC17 in Arabidopsis thaliana enhanced drought tolerance. VaNAC17-expressing Arabidopsis plants showed decreased reactive oxygen species (ROS) accumulation compared to wild-type plants under drought conditions. RNA-seq analysis indicated that VaNAC17 expression increased the transcription of downstream stress-responsive genes after 5 days of drought treatment, especially genes involved in jasmonic acid (JA) biosynthesis (such as LOX3, AOC1 and OPR3) and signaling (such as MYC2, JAZ1, VSP1 and CORI3) pathways. Endogenous JA levels increased in VaNAC17-OE plants under drought stress. Taken together, these results indicate that VaNAC17 plays a positive role in drought tolerance by modulating endogenous JA biosynthesis and ROS scavenging.
学科主题	Plant Sciences
电子版国际标准刊号	1432-203X
出版地	NEW YORK
WOS关键词	STRESS-RESPONSE ; ABSCISIC-ACID ; GENE FAMILY ; OSMOTIC-STRESS ; PLANT STRESS ; NAC ; EXPRESSION ; GROWTH ; DEFENSE ; REGULATOR
WOS研究方向	Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000516940700001
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31672132] ; Youth Innovation Promotion Association of Chinese Academy of Sciences [2015281] ; Grape Breeding Project of Ningxia [NXNYYZ201502]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21890]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Sino Africa Joint Res Ctr, Wuhan Bot Garden, Wuhan, Peoples R China 2.Chinese Acad Sci, Ctr Econ Bot, Core Bot Gardens, Wuhan, Peoples R China 3.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Lab Plant Resources, Inst Bot, Beijing, Peoples R China 4.Guangdong Acad Forestry, Guangdong Prov Key Lab Silviculture Protect & Uti, Guangzhou, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Su, Lingye,Fang, Linchuan,Zhu, Zhenfei,et al. The transcription factor VaNAC17 from grapevine (Vitis amurensis) enhances drought tolerance by modulating jasmonic acid biosynthesis in transgenic Arabidopsis[J]. PLANT CELL REPORTS,2020,39(5):621-634.
APA	Su, Lingye.,Fang, Linchuan.,Zhu, Zhenfei.,Zhang, Langlang.,Sun, Xiaoming.,...&Xin, Haiping.(2020).The transcription factor VaNAC17 from grapevine (Vitis amurensis) enhances drought tolerance by modulating jasmonic acid biosynthesis in transgenic Arabidopsis.PLANT CELL REPORTS,39(5),621-634.
MLA	Su, Lingye,et al."The transcription factor VaNAC17 from grapevine (Vitis amurensis) enhances drought tolerance by modulating jasmonic acid biosynthesis in transgenic Arabidopsis".PLANT CELL REPORTS 39.5(2020):621-634.