Genome-wide analyses reveals a glucosyltransferase involved in rutin and emodin glucoside biosynthesis in tartary buckwheat

doi:10.1016/j.foodchem.2020.126478

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	Genome-wide analyses reveals a glucosyltransferase involved in rutin and emodin glucoside biosynthesis in tartary buckwheat
	Yin, Qinggang ; Han, Xiaoyan 2; Han, Zongxian 3; Chen, Qingfu 1; Shi, Yuhua ; Gao, Han 3; Zhang, Tianyuan ; Dong, Gangqiang 5; Xiong, Chao ; Song, Chi
刊名	FOOD CHEMISTRY
	2020
卷号	318
关键词	Tartary buckwheat Rutin Emodin Glucoside Biosynthesis Glucosyltransferase Genome-wide
ISSN号	0308-8146
DOI	10.1016/j.foodchem.2020.126478
文献子类	Article
英文摘要	With people's increasing needs for health concern, rutin and emodin in tartary buckwheat have attracted much attention for their antioxidant, anti-diabetic and reducing weight function. However, the biosynthesis of rutin and emodin in tartary buckwheat is still unclear; especially their later glycosylation contributing to make them more stable and soluble is uncovered. Based on tartary buckwheat' genome, the gene structures of 106 UGTs were analyzed; 21 candidate FtUGTs were selected to enzymatic test by comparing their transcript patterns. Among them, FtUGT73BE5 and other 4 FtUGTs were identified to glucosylate flavonol or emodin in vitro; especially rFtUGT73BE5 could catalyze the glucosylation of all tested flavonoids and emodin. Furthermore, the identical in vivo functions of FtUGT73BE5 were demonstrated in tartary buckwheat hairy roots. The transcript profile of FtUGT73BE5 was consistent with the accumulation trend of rutin in plant; this gene may relate to antiadversity for its transcripts were up-regulated by MeJA, and repressed by ABA.
学科主题	Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
电子版国际标准刊号	1873-7072
出版地	OXFORD
WOS关键词	FAGOPYRUM-TATARICUM GAERTN. ; FUNCTIONAL-CHARACTERIZATION ; MOLECULAR-CLONING ; SEED COAT ; GLYCOSYLTRANSFERASE ; IDENTIFICATION ; 3-O-GLUCOSYLTRANSFERASE ; ANTHRAQUINONES ; GLYCOSYLATION ; EXPRESSION
WOS研究方向	Chemistry ; Food Science & Technology ; Nutrition & Dietetics
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000520858000032
资助机构	Beijing Natural Science Foundation of ChinaBeijing Natural Science Foundation [7192138] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81703647, 31471562] ; Fundamental Research Funds for the Central public welfare research institutes [ZZ13-YQ-097] ; National Key R&D Program of China [2019YFC1711100] ; National Science and Technology Major Project Creation of Major New Drugs [2019ZX09201005-006-004, 2019ZX09731-002]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21852]
专题	植物园
作者单位	1.Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, 122 Lo Lion Rd, Wuhan 430070, Hubei, Peoples R China 2.China Acad Chinese Med Sci, Inst Chinese Mat Med, Key Lab Beijing Identificat & Safety Evaluat Chin, Beijing 100700, Peoples R China 3.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 4.Amway China Bot R&D Ctr, Wuxi 214115, Jiangsu, Peoples R China 5.Guizhou Normal Univ, Res Ctr Buckwheat Ind Technol, Baoshan Beilu 116, Guiyang 550001, Peoples R China
推荐引用方式 GB/T 7714	Yin, Qinggang,Han, Xiaoyan,Han, Zongxian,et al. Genome-wide analyses reveals a glucosyltransferase involved in rutin and emodin glucoside biosynthesis in tartary buckwheat[J]. FOOD CHEMISTRY,2020,318.
APA	Yin, Qinggang.,Han, Xiaoyan.,Han, Zongxian.,Chen, Qingfu.,Shi, Yuhua.,...&Chen, Shilin.(2020).Genome-wide analyses reveals a glucosyltransferase involved in rutin and emodin glucoside biosynthesis in tartary buckwheat.FOOD CHEMISTRY,318.
MLA	Yin, Qinggang,et al."Genome-wide analyses reveals a glucosyltransferase involved in rutin and emodin glucoside biosynthesis in tartary buckwheat".FOOD CHEMISTRY 318(2020).