The F-box protein EST1 modulates salt tolerance in Arabidopsis by regulating plasma membrane Na+/H+ antiport activity

doi:10.1016/j.jplph.2020.153217

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	The F-box protein EST1 modulates salt tolerance in Arabidopsis by regulating plasma membrane Na+/H+ antiport activity
	Liu, Jie ; Lin, Qing Fang 5; Qi, Shi Lian 3; Feng, Xuan Jun 4; Han, Hui Ling 5; Xu, Tao 2; Hua, Xue Jun 2
刊名	JOURNAL OF PLANT PHYSIOLOGY
	2020
卷号	251
关键词	F-box protein Salt tolerance Na+/H+ antiporter Mitogen-activated protein kinase Arabidopsis thaliana
ISSN号	0176-1617
DOI	10.1016/j.jplph.2020.153217
文献子类	Article
英文摘要	F-box protein, one of the building blocks of the SCF complex, functions in substrate recognition of the SCF subtype of E3 ubiquitin ligase. However, the role of F-box protein in salt stress is largely elusive in plants. Here, we report the characterization of an Arabidopsis salt-tolerant mutant est1 with significantly reduced sodium content and higher Na+/H+ antiporter activity after NaCl treatment compared to the wild-type. Over-expression of EST1 resulted in increased sensitivity to salt stress, suggesting that EST1 may act as a negative regulator for salt tolerance in Arabidopsis. EST1 encodes an F-box protein, which interacts with ASK4, ASK14, and ASK18, and is likely targeted to the endoplasmic reticulum. In addition, EST1 interacts with MKK4 and negatively regulates MKK4 protein levels and the activity of the plasma membrane Na+/H+ antiporter. Our findings demonstrate the existence of an EST1-MKK4 module that mediates salt sensitivity by regulating the activity of the plasma membrane Na+/H+ antiporter. These results provide important information for engineering salt-tolerant crops.
学科主题	Plant Sciences
电子版国际标准刊号	1618-1328
出版地	MUNICH
WOS关键词	STRESS-RESPONSE ; ION HOMEOSTASIS ; DROUGHT STRESS ; KINASE ; THALIANA ; SOS1 ; PATHWAY ; SCF ; MECHANISMS ; EXPRESSION
WOS研究方向	Plant Sciences
语种	英语
出版者	ELSEVIER GMBH
WOS记录号	WOS:000550450400008
资助机构	National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31770299, 31701926] ; Beijing Municipal Natural Science FoundationBeijing Natural Science Foundation [5182020]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21672]
专题	中科院北方资源植物重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Zhejiang Sci Tech Univ, Key Lab Plant Secondary Metab & Regulat Zhejiang, Coll Life Sci & Med, Hangzhou 310018, Zhejiang, Peoples R China 4.Jiangsu Normal Univ, Key Lab Biotechnol Med Plant Jiangsu Prov, Xuzhou 221116, Jiangsu, Peoples R China 5.Sichuan Agr Univ, Maize Res Inst, Wenjiang 611130, Sichuan, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jie,Lin, Qing Fang,Qi, Shi Lian,et al. The F-box protein EST1 modulates salt tolerance in Arabidopsis by regulating plasma membrane Na+/H+ antiport activity[J]. JOURNAL OF PLANT PHYSIOLOGY,2020,251.
APA	Liu, Jie.,Lin, Qing Fang.,Qi, Shi Lian.,Feng, Xuan Jun.,Han, Hui Ling.,...&Hua, Xue Jun.(2020).The F-box protein EST1 modulates salt tolerance in Arabidopsis by regulating plasma membrane Na+/H+ antiport activity.JOURNAL OF PLANT PHYSIOLOGY,251.
MLA	Liu, Jie,et al."The F-box protein EST1 modulates salt tolerance in Arabidopsis by regulating plasma membrane Na+/H+ antiport activity".JOURNAL OF PLANT PHYSIOLOGY 251(2020).