Crystal structure of Arabidopsis thaliana cytidine deaminase

doi:10.1016/j.bbrc.2020.06.084

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	Crystal structure of Arabidopsis thaliana cytidine deaminase
	Wang, Jia 3; Guo, Qi 3; Liu, Lin 2; Wang, Xiao 2
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2020
卷号	529 期号:3 页码:659-665
关键词	Pyrimidine metabolism RNA editing Crystallography Catalytic mechanism
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2020.06.084
文献子类	Article
英文摘要	Cytidine deaminase (CDA) catalyzes the (deoxy)cytidine deamination to (deoxy)uridine, which involves in the catabolic and salvage pathways of pyrimidine nucleotides in plants. CDA serves as a prototype of the cytidine deaminase superfamily that contains a number of RNA editing enzymes. Arabidopsis thaliana has only one functional CDA, AtCDA1. We solved the crystal structures of AtCDA1, which is a dimeric zinccontaining enzyme and each protomer consists of an N-terminal zinc-binding catalytic domain and a C-terminal non-catalytic domain. Both domains adopt a typical alpha/beta/alpha sandwich fold. In vitro biochemical assays showed that the ribose moiety of cytidine is required for ligand binding, and structural analyses revealed a conserved catalytic mechanism is adopted by AtCDA1. (C) 2020 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Biophysics
电子版国际标准刊号	1090-2104
出版地	SAN DIEGO
WOS关键词	FAMILY PROTEINS ; CHLOROPHYLL-A ; PYRIMIDINE ; ENZYME ; MODEL
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000555780500022
资助机构	National Key R&D Program of China [2017YFA0503703] ; China Postdoctoral Science FundChina Postdoctoral Science Foundation [2018M632517] ; Provincial Natural Science Foundation of Anhui [1908085QC129]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21644]
专题	中科院光生物学重点实验室
作者单位	1.Anhui Univ, Sch Life Sci, Duxing North Bldg,Rm B305,111 Jiulong Rd, Hefei 230601, Anhui, Peoples R China 2.Univ Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Bot, CAS Key Lab Photobiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jia,Guo, Qi,Liu, Lin,et al. Crystal structure of Arabidopsis thaliana cytidine deaminase[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2020,529(3):659-665.
APA	Wang, Jia,Guo, Qi,Liu, Lin,&Wang, Xiao.(2020).Crystal structure of Arabidopsis thaliana cytidine deaminase.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,529(3),659-665.
MLA	Wang, Jia,et al."Crystal structure of Arabidopsis thaliana cytidine deaminase".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 529.3(2020):659-665.