An innovative artificial photosystem II constructed from PSII core of Thermosynechococcus vulcanus and LHCII of Pisum sativum - A new approach for studying the function of photosynthetic antenna

doi:10.1016/j.plaphy.2020.05.030

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	An innovative artificial photosystem II constructed from PSII core of Thermosynechococcus vulcanus and LHCII of Pisum sativum - A new approach for studying the function of photosynthetic antenna
	Li, Ling 1; Li, Fei 1; Wang, Yue 1; Yang, Chunhong 1,4; Lu, Congming 3; Liu, Cheng 1; Sun, Ruixue 4
刊名	PLANT PHYSIOLOGY AND BIOCHEMISTRY
	2020
卷号	154 页码:160-170
关键词	Photosystem II LHCII Proteoliposome Energy transfer
ISSN号	0981-9428
DOI	10.1016/j.plaphy.2020.05.030
文献子类	Article
英文摘要	In photosynthesis, the antenna system captures solar energy and transfers the excitations to photosystem II (PSII) core complex where charge separation, water splitting and oxygen evolution occur. In the evolution of photosynthesis from aquatic to terrestrial environments, the structure of PSII core complex was highly conserved while a variety of antenna forms became differentiated. In order to study the principles for energy transport from antenna to the PSII reaction center, we have explored whether the major light harvesting complex of PSII (LHCII) of higher plants can transfer energy to the cyanobacteria PSII core complexes (CC). For this purpose, LHCII from pea and CC from Thermosynechococcus vulcanus were isolated and co-reconstituted into liposome at LHCII:CC molar ratios of 2:1, 4:1 and 6:1, respectively. Chemical-cross linking followed by LC-MS/MS analysis confirmed the biochemical interaction between LHCII and CC in the liposome membrane. The analyses of 77 K fluorescence emission spectra and antenna cross section of PSII indicated that LHCII can transfer energy directly to the cyanobacterial CC. The study has laid the basis for further research on the mechanism of energy transfer from LHCII to PSII CC. This result may also open a new possibility for design and development of new artificial PSII in the application of solar energy conversion.
学科主题	Plant Sciences
出版地	ISSY-LES-MOULINEAUX
WOS关键词	LIGHT-HARVESTING COMPLEX ; PROTEIN COMPLEXES ; CRYSTAL-STRUCTURE ; ENERGY-TRANSFER ; EVOLUTION ; IDENTIFICATION ; STABILITY ; ISOFORMS ; MUTANTS ; PLANTS
WOS研究方向	Plant Sciences
语种	英语
出版者	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
WOS记录号	WOS:000566818900016
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31570236, 31370275] ; National Key R&D Program of China [2017YFA0503701]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21594]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing Bot Garden, Beijing 100093, Peoples R China 2.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China 3.Chinese Acad Sci, Qingdao Inst, Shanghai Inst Technol Phys, Binhai Rd 50, Qingdao 264000, Peoples R China 4.Univ Chinese Acad Sci, Yuquan Rd 19A, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Ling,Li, Fei,Wang, Yue,et al. An innovative artificial photosystem II constructed from PSII core of Thermosynechococcus vulcanus and LHCII of Pisum sativum - A new approach for studying the function of photosynthetic antenna[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2020,154:160-170.
APA	Li, Ling.,Li, Fei.,Wang, Yue.,Yang, Chunhong.,Lu, Congming.,...&Sun, Ruixue.(2020).An innovative artificial photosystem II constructed from PSII core of Thermosynechococcus vulcanus and LHCII of Pisum sativum - A new approach for studying the function of photosynthetic antenna.PLANT PHYSIOLOGY AND BIOCHEMISTRY,154,160-170.
MLA	Li, Ling,et al."An innovative artificial photosystem II constructed from PSII core of Thermosynechococcus vulcanus and LHCII of Pisum sativum - A new approach for studying the function of photosynthetic antenna".PLANT PHYSIOLOGY AND BIOCHEMISTRY 154(2020):160-170.