SlFERL Interacts with S-Adenosylmethionine Synthetase to Regulate Fruit Ripening

doi:10.1104/pp.20.01203

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	SlFERL Interacts with S-Adenosylmethionine Synthetase to Regulate Fruit Ripening
	Ji, Dongchao 2; Cui, Xiaomin 2; Qin, Guozheng ; Chen, Tong ; Tian, Shiping 1,2
刊名	PLANT PHYSIOLOGY
	2020
卷号	184 期号:4 页码:2168-2181
ISSN号	0032-0889
DOI	10.1104/pp.20.01203
文献子类	Article
英文摘要	The tomato membrane protein SlFERL regulates fruit ripening via modulating ethylene production. Fruit ripening is a complex and genetically programmed process modulated by transcription factors, hormones, and other regulators. However, the mechanism underlying the regulatory loop involving the membrane-protein targets of RIPENING-INHIBITOR (RIN) remains poorly understood. To unravel the function of tomato (Solanum lycopersicum) FERONIA Like (SlFERL), a putative MADS-box transcription factor target gene, we investigated and addressed the significance of SlFERL in fruit ripening by combining reverse genetics, biochemical, and cytological analyses. Here, we report that RIN and Tomato AGAMOUS-LIKE1 (TAGL1) directly bind to the promoter region of SlFERL and further activate its expression transcriptionally, suggesting a potential role of SlFERL in fruit ripening. Overexpression of SlFERL significantly accelerated the ripening process of tomato fruit, whereas RNA interference knockdown of SlFERL resulted in delayed fruit ripening. Moreover, a surface plasmon resonance assay coupled with tandem mass spectrometry and a protein interaction assay revealed that SlFERL interacts with the key enzyme S-adenosyl-Met synthetase 1 (SlSAMS1) in the ethylene biosynthesis pathway, leading to increased S-adenosyl-Met accumulation and elevated ethylene production. Thus, SlFERL serves as a positive regulator of ethylene production and fruit ripening. This study provides clues to the molecular regulatory networks underlying fruit ripening.
学科主题	Plant Sciences
电子版国际标准刊号	1532-2548
出版地	ROCKVILLE
WOS关键词	ADENOSYL-L-METHIONINE ; ETHYLENE BIOSYNTHESIS ; RECEPTOR KINASES ; INHIBITOR RIN ; TOMATO ; FERONIA ; ARABIDOPSIS ; GENE ; REVEAL ; IDENTIFICATION
WOS研究方向	Plant Sciences
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000601303200046
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31930086, 31925035, 32072637]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21490]
专题	中科院北方资源植物重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Key Lab Post Harvest Handling Fruits, Minist Agr, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Ji, Dongchao,Cui, Xiaomin,Qin, Guozheng,et al. SlFERL Interacts with S-Adenosylmethionine Synthetase to Regulate Fruit Ripening[J]. PLANT PHYSIOLOGY,2020,184(4):2168-2181.
APA	Ji, Dongchao,Cui, Xiaomin,Qin, Guozheng,Chen, Tong,&Tian, Shiping.(2020).SlFERL Interacts with S-Adenosylmethionine Synthetase to Regulate Fruit Ripening.PLANT PHYSIOLOGY,184(4),2168-2181.
MLA	Ji, Dongchao,et al."SlFERL Interacts with S-Adenosylmethionine Synthetase to Regulate Fruit Ripening".PLANT PHYSIOLOGY 184.4(2020):2168-2181.