Quantitative Proteomics and Cytology of Rice Pollen Sterol-Rich Membrane Domains Reveals Pre-established Cell Polarity Cues in Mature Pollen

doi:10.1021/acs.jproteome.7b00852

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	Quantitative Proteomics and Cytology of Rice Pollen Sterol-Rich Membrane Domains Reveals Pre-established Cell Polarity Cues in Mature Pollen
	Han, Bing 2,3; Yang, Ning 2; Pu, Hai 1; Wang, Tai 2
刊名	JOURNAL OF PROTEOME RESEARCH
	2018
卷号	17 期号:4 页码:1532-1546
关键词	cell asymmetry filipin sterol-rich membrane microdomains label-free quantitative proteomics methyl-beta-cyclodextrin (M beta CD) polar growth pollen tube rice pollen
ISSN号	1535-3893
DOI	10.1021/acs.jproteome.7b00852
文献子类	Article
英文摘要	Cell polarity is essential for generating diverse cell functions. The underlying mechanisms of how a cell establishes, maintains, and changes its polarity are poorly understood. Recently, sterol-rich membrane microdomains are found to be associated with these processes. However, both its exact characteristics and importance are still unclear. Here we show microdomains change dynamically in developing and germinating rice pollen with selective enrichment in the aperture and the tip of newly born pollen tubes by use of the sterol-specific probe filipin. Using the sterol extraction sensitivities of microdomain proteins and quantitative proteomics, we identified 237 microdomain-associated proteins from 934 identified pollen detergent resistant membrane proteins. This proteome includes almost all of the known key regulators comprising the polar growth network, and it shows more similarity to front-back polarized HeLa cells than nonpolarized Arabidopsis suspension cells. We immunolocalize flotilin-like protein, a representative of these sterol-dependent proteins and directly visualize microdomains in pollen. These results indicate the presence of microdomains in pollen and preestablished cell polarity around the aperture during pollen maturation. Our findings reveal an atlas of the microdomain-associated proteome in pollen. This work provides useful resources and knowledge needed to further dissect the mechanisms for the establishment and maintenance of cell polarity.
学科主题	Biochemical Research Methods
电子版国际标准刊号	1535-3907
出版地	WASHINGTON
WOS关键词	DETERGENT-RESISTANT MEMBRANES ; EPITHELIAL-MESENCHYMAL TRANSITION ; MALE GAMETOPHYTE DEVELOPMENT ; RAFT-LIKE MICRODOMAINS ; PLASMA-MEMBRANE ; LIPID RAFTS ; TIP GROWTH ; TUBE GROWTH ; DEPENDENT ENDOCYTOSIS ; ARABIDOPSIS-THALIANA
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000429886600018
资助机构	Chinese Ministry of Science and TechnologyMinistry of Science and Technology, China [2013CB945101] ; Chinese Academy of SciencesChinese Academy of Sciences [KSCX2-YW-N-026]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/20661]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Bruker Dalton Inc China, Beijing 100081, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Vanderbilt Univ, Dept Mol Physiol & Biophys, Nashville, TN 37232 USA
推荐引用方式 GB/T 7714	Han, Bing,Yang, Ning,Pu, Hai,et al. Quantitative Proteomics and Cytology of Rice Pollen Sterol-Rich Membrane Domains Reveals Pre-established Cell Polarity Cues in Mature Pollen[J]. JOURNAL OF PROTEOME RESEARCH,2018,17(4):1532-1546.
APA	Han, Bing,Yang, Ning,Pu, Hai,&Wang, Tai.(2018).Quantitative Proteomics and Cytology of Rice Pollen Sterol-Rich Membrane Domains Reveals Pre-established Cell Polarity Cues in Mature Pollen.JOURNAL OF PROTEOME RESEARCH,17(4),1532-1546.
MLA	Han, Bing,et al."Quantitative Proteomics and Cytology of Rice Pollen Sterol-Rich Membrane Domains Reveals Pre-established Cell Polarity Cues in Mature Pollen".JOURNAL OF PROTEOME RESEARCH 17.4(2018):1532-1546.