The pH-responsive PacC transcription factor plays pivotal roles in virulence and patulin biosynthesis in Penicillium expansum

doi:10.1111/1462-2920.14453

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	The pH-responsive PacC transcription factor plays pivotal roles in virulence and patulin biosynthesis in Penicillium expansum
	Chen, Yong 3; Li, Boqiang ; Xu, Xiaodi 3; Zhang, Zhanquan ; Tian, Shiping 1,3
刊名	ENVIRONMENTAL MICROBIOLOGY
	2018
卷号	20 期号:11 页码:4063-4078
ISSN号	1462-2912
DOI	10.1111/1462-2920.14453
文献子类	Article
英文摘要	The PacC (loss or reduction in phosphatase activity at acid but not at alkaline pH [Pac]) transcription factor regulates environmental adaptation, secondary metabolism and virulence in many fungal pathogens. Here, we report the functions of PacC in Penicillium expansum, a postharvest pathogenic fungus in horticultural crops, and ascertain that the gene expression and proteolytic processing of PePacC are strictly pH-dependent. Loss of PePacC resulted in an obvious decrease in growth and conidiation of P. expansum cultured in both acidic and alkaline conditions. The Delta PePacC mutant lost the ability of patulin production at pH values above 6.0 because expressions of all the genes in patulin cluster were significantly down-regulated. Additionally, virulence of the Delta PePacC mutant was obviously reduced in pear and apple fruits. Proteome analysis revealed that PePacC could function as an activator or repressor for different target proteins, including calreticulin (PeCRT) and sulfate adenylyltransferase (PeSAT), which were further proved to be involved in virulence of P. expansum. Our results demonstrate important roles for PePacC in patulin biosynthesis via limiting expressions of the genes in the cluster, and in pathogenesis via mediating a known virulence factor glucose oxidase (PeGOD) and new virulence factors, such as PeCRT and PeSAT.
学科主题	Microbiology
电子版国际标准刊号	1462-2920
出版地	HOBOKEN
WOS关键词	AMBIENT PH ; GENE-EXPRESSION ; MOLECULAR CHARACTERIZATION ; REGULATORY GENE ; PATHOGENICITY ; APPLE ; ACIDIFICATION ; COLONIZATION ; ACCUMULATION ; INVOLVEMENT
语种	英语
出版者	WILEY
WOS记录号	WOS:000450357400013
资助机构	National Key R&D Program of China [2016YFD0400902] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530057, 31722043]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/20532]
专题	中科院北方资源植物重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Minist Educ, Biotechnol & Bioresources Utilizat, Dalian 116600, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yong,Li, Boqiang,Xu, Xiaodi,et al. The pH-responsive PacC transcription factor plays pivotal roles in virulence and patulin biosynthesis in Penicillium expansum[J]. ENVIRONMENTAL MICROBIOLOGY,2018,20(11):4063-4078.
APA	Chen, Yong,Li, Boqiang,Xu, Xiaodi,Zhang, Zhanquan,&Tian, Shiping.(2018).The pH-responsive PacC transcription factor plays pivotal roles in virulence and patulin biosynthesis in Penicillium expansum.ENVIRONMENTAL MICROBIOLOGY,20(11),4063-4078.
MLA	Chen, Yong,et al."The pH-responsive PacC transcription factor plays pivotal roles in virulence and patulin biosynthesis in Penicillium expansum".ENVIRONMENTAL MICROBIOLOGY 20.11(2018):4063-4078.