Inhibitory effects of methyl thujate on mycelial growth of Botrytis cinerea and possible mechanisms

doi:10.1016/j.postharvbio.2018.04.003

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	Inhibitory effects of methyl thujate on mycelial growth of Botrytis cinerea and possible mechanisms
	Ji, Dongchao 4; Chen, Tong ; Ma, Danying 4; Liu, Jialei 1; Xu, Yong ; Tian, Shiping 2,4
刊名	POSTHARVEST BIOLOGY AND TECHNOLOGY
	2018
卷号	142 页码:46-54
关键词	Botrytis cinerea Methyl thujate Reactive oxygen species Membrane integrity
ISSN号	0925-5214
DOI	10.1016/j.postharvbio.2018.04.003
文献子类	Article
英文摘要	Methyl thujate, a monoterpenoid substance, was effective in controlling postharvest gray mold caused by Botrytis cinerea on apple fruit, during application in aqueous solution. Methyl thujate strongly inhibited spore germination, germ tube elongation and mycelial spreading of B. cinerea in the culture medium, and the inhibition on fruit decay was closely correlated with methyl thujate concentrations. By means of propidium iodide and fluorescein diacetate staining, loss of membrane integrity and cell viability in B. cinerea was detected after methyl thujate treatment. Furthermore, methyl thujate led to cytoplasmic accumulation of reactive oxygen species, leakage of cellular constituents and variations in cell wall component deposition in B. cinerea hyphae, as the genes encoded subunits for NADPH oxidase were synergistically upregulated, whereas the genes involved in cell wall modeling and virulence responded differentially. Taken together, these data suggest that the mechanisms by which methyl thujate decreased fruit decay may be directly attributed to the induced ROS burst, breakdown of cell membrane integrity and loss of cytoplasmic contents from the hyphae.
学科主题	Agronomy ; Food Science & Technology ; Horticulture
电子版国际标准刊号	1873-2356
出版地	AMSTERDAM
WOS关键词	PENICILLIUM-EXPANSUM ; GENE-EXPRESSION ; NADPH OXIDASE ; GRAY MOLD ; IN-VITRO ; VIRULENCE ; PATHOGENICITY ; COMPONENT ; SUBUNIT ; DISEASE
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000430885400006
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530057, 30672210] ; Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2015BAD16B01-3, 2016YFD0400903, 2017YFD0401301]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/20515]
专题	中科院北方资源植物重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Phys & Chem Tech, Beijing 100093, Peoples R China 3.Minist Agr, Key Lab Postharvest Handling Fruits, Beijing 100093, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Ji, Dongchao,Chen, Tong,Ma, Danying,et al. Inhibitory effects of methyl thujate on mycelial growth of Botrytis cinerea and possible mechanisms[J]. POSTHARVEST BIOLOGY AND TECHNOLOGY,2018,142:46-54.
APA	Ji, Dongchao,Chen, Tong,Ma, Danying,Liu, Jialei,Xu, Yong,&Tian, Shiping.(2018).Inhibitory effects of methyl thujate on mycelial growth of Botrytis cinerea and possible mechanisms.POSTHARVEST BIOLOGY AND TECHNOLOGY,142,46-54.
MLA	Ji, Dongchao,et al."Inhibitory effects of methyl thujate on mycelial growth of Botrytis cinerea and possible mechanisms".POSTHARVEST BIOLOGY AND TECHNOLOGY 142(2018):46-54.