Calcium imaging in Arabidopsis pollen cells using G-CaMP5

doi:10.1111/jipb.12642

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	Calcium imaging in Arabidopsis pollen cells using G-CaMP5
	Diao, Min 2,3; Qu, Xiaolu 2; Huang, Shanjin 2
刊名	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
	2018
卷号	60 期号:9 页码:897-906
ISSN号	1672-9072
DOI	10.1111/jipb.12642
文献子类	Article
英文摘要	Calcium (Ca2+) signaling has been implicated in pollen germination and pollen tube growth. To date, however, we still know very little about how exactly Ca2+ signaling links to various physiological subcellular processes during pollen germination and pollen tube growth. Given that Ca2+ signaling is tightly related to the cytosolic concentration and dynamics of Ca2+, it is vital to trace the dynamic changes in Ca2+ levels in order to decode Ca2+ signaling. Here, we demonstrate that G-CaMP5 serves well as an indicator for monitoring cytosolic Ca2+ dynamics in pollen cells. Using this probe, we show that cytosolic Ca2+ changes dramatically during pollen germination, and, as reported previously, Ca2+ forms a tip-focused gradient in the pollen tube and undergoes oscillation in the tip region during pollen tube growth. In particular, using G-CaMP5 allowed us to capture the dynamic changes in the cytosolic Ca2+ concentration ([Ca2+](cyt)) in pollen tubes in response to various exogenous treatments. Our data suggest that G-CaMP5 is a suitable probe for monitoring the dynamics of [Ca2+](cyt) in pollen cells.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
电子版国际标准刊号	1744-7909
出版地	HOBOKEN
WOS关键词	GREEN FLUORESCENT PROTEIN ; TUBE TIP GROWTH ; PLASMA-MEMBRANE ; NEURAL ACTIVITY ; CA2+ CHANNELS ; GRADIENT ; CALMODULIN ; GERMINATION ; DYNAMICS ; INFLUX
语种	英语
出版者	WILEY
WOS记录号	WOS:000443808500012
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471266, 31671390] ; Tsinghua-Peking Joint Center for Life Sciences
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/20423]
专题	中科院植物分子生理学重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Tsinghua Univ, Sch Life Sci, Ctr Plant Biol, Beijing 100084, Peoples R China
推荐引用方式 GB/T 7714	Diao, Min,Qu, Xiaolu,Huang, Shanjin. Calcium imaging in Arabidopsis pollen cells using G-CaMP5[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2018,60(9):897-906.
APA	Diao, Min,Qu, Xiaolu,&Huang, Shanjin.(2018).Calcium imaging in Arabidopsis pollen cells using G-CaMP5.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,60(9),897-906.
MLA	Diao, Min,et al."Calcium imaging in Arabidopsis pollen cells using G-CaMP5".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 60.9(2018):897-906.