OsMTOPVIB is required for meiotic bipolar spindle assembly

doi:10.1073/pnas.1821315116

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院植物分子生理学重点实验室

	OsMTOPVIB is required for meiotic bipolar spindle assembly
	Xue, Zhihui 3,4; Liu, Changzhen 3; Shi, Wenqing 3; Miao, Yongjie 3; Shen, Yi 3; Tang, Ding 3; Li, Yafei 3; You, Aiqing 1; Xu, Yunyuan 4; Chong, Kang 4
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2019
卷号	116 期号:32 页码:15967-15972
关键词	rice meiosis spindle assembly kinetochore
ISSN号	0027-8424
DOI	10.1073/pnas.1821315116
文献子类	Article
英文摘要	The organization of microtubules into a bipolar spindle is essential for chromosome segregation. Both centrosome and chromat-independent spindle assembly mechanisms are well studied in mouse, Drosophila melanogaster, and Xenopus oocytes; however, the mechanism of bipolar spindle assembly in plant meiosis remains elusive. According to our observations of microtubule assembly in Oryza sativa, Zea mays, Arabidopsis thaliana, and Solanum lycopersicum, we propose that a key step of plant bipolar spindle assembly is the correction of the multipolar spindle into a bipolar spindle at metaphase I. The multipolar spindles failed to transition into bipolar ones in OsmtopVIB with the defect in double-strand break (DSB) formation. However, bipolar spindles were normally assembled in several other mutants lacking DSB formation, such as Osspo11-1, pair2, and crc1, indicating that bipolar spindle assembly is independent of DSB formation. We further revealed that the mono-orientation of sister kinetochores was prevalent in OsmtopVIB, whereas biorientation of sister kinetochores was frequently observed in Osspo11-1, pair2, and crc1. In addition, mutations of the cohesion subunit OsREC8 resulted in biorientation of sister kinetochores as well as bipolar spindles even in the background of OsmtopVIB. Therefore, we propose that biorientation of the kinetochore is required for bipolar spindle assembly in the absence of homologous recombination.
学科主题	Multidisciplinary Sciences
出版地	WASHINGTON
WOS关键词	GAMMA-TUBULIN ; RECOMBINATION INITIATION ; MONOPOLAR ORIENTATION ; CHROMATID COHESION ; SELF-ORGANIZATION ; PLANT-CELLS ; MEIOSIS ; CHROMOSOME ; MICROTUBULES ; DIVISION
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATL ACAD SCIENCES
WOS记录号	WOS:000478971900037
资助机构	National Key Research and Development Program of China [2016YFD0100901] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31771363]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/19836]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Hubei Acad Agr Sci, Food Crops Inst, Wuhan 430064, Hubei, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Xue, Zhihui,Liu, Changzhen,Shi, Wenqing,et al. OsMTOPVIB is required for meiotic bipolar spindle assembly[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2019,116(32):15967-15972.
APA	Xue, Zhihui.,Liu, Changzhen.,Shi, Wenqing.,Miao, Yongjie.,Shen, Yi.,...&Cheng, Zhukuan.(2019).OsMTOPVIB is required for meiotic bipolar spindle assembly.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,116(32),15967-15972.
MLA	Xue, Zhihui,et al."OsMTOPVIB is required for meiotic bipolar spindle assembly".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116.32(2019):15967-15972.