IDD16 negatively regulates stomatal initiation via trans-repression of SPCH in Arabidopsis

doi:10.1111/pbi.13070

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	IDD16 negatively regulates stomatal initiation via trans-repression of SPCH in Arabidopsis
	Qi, Shi-Lian ; Lin, Qing-Fang ; Feng, Xuan-Jun ; Han, Hui-Ling ; Liu, Jie 9; Zhang, Liu 1; Wu, Shuang 4; Le, Jie 2,9; Blumwald, Eduardo 8; Hua, Xue-Jun
刊名	PLANT BIOTECHNOLOGY JOURNAL
	2019
卷号	17 期号:7 页码:1446-1457
关键词	IDD16 SPCH stomatal development water use efficiency drought tolerance Arabidopsis thaliana
ISSN号	1467-7644
DOI	10.1111/pbi.13070
文献子类	Article
英文摘要	In Arabidopsis, the initiation and proliferation of stomatal lineage cells is controlled by SPEECHLESS (SPCH). Phosphorylation of SPCH at the post-translational level has been reported to regulate stomatal development. Here we report that IDD16 acts as a negative regulator for stomatal initiation by directly regulating SPCH transcription. In Arabidopsis, IDD16 overexpression decreased abaxial stomatal density in a dose-dependent manner. Time course analysis revealed that the initiation of stomatal precursor cells in the IDD16-OE plants was severely inhibited. Consistent with these findings, the transcription of SPCH was greatly repressed in the IDD16-OE plants. In contrast, IDD16-RNAi transgenic line resulted in enhanced stomatal density, suggesting that IDD16 is an intrinsic regulator of stomatal development. ChIP analysis indicated that IDD16 could directly bind to the SPCH promoter. Furthermore, Arabidopsis plants overexpressing IDD16 exhibited significantly increased drought tolerance and higher integrated water use efficiency (WUE) due to reduction in leaf transpiration. Collectively, our results established that IDD16 negatively regulates stomatal initiation via trans-repression of SPCH, and thus provide a practical tool for increasing plant WUE through the manipulation of IDD16 expression.
学科主题	Biotechnology & Applied Microbiology ; Plant Sciences
电子版国际标准刊号	1467-7652
出版地	HOBOKEN
WOS关键词	WATER-USE EFFICIENCY ; ASYMMETRIC CELL DIVISIONS ; DROUGHT TOLERANCE ; TRANSCRIPTION FACTOR ; PLANT-RESPONSES ; ABSCISIC-ACID ; DENSITY ; FATE ; EXPRESSION ; SPEECHLESS
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
语种	英语
出版者	WILEY
WOS记录号	WOS:000471831200023
资助机构	National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31770299, 31771515, 31271463]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/19778]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Plant Mol Physiol, CAS Ctr Excellence Mol Plant Sci, Inst Bot, Beijing, Peoples R China 2.Fujian Agr & Forestry Univ, Coll Crop Sci, Fuzhou, Fujian, Peoples R China 3.Fujian Agr & Forestry Univ, Coll Hort, Fuzhou, Fujian, Peoples R China 4.Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou, Zhejiang, Peoples R China 5.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing, Peoples R China 6.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 7.Univ Calif Davis, Dept Plant Sci, Davis, CA USA 8.Univ Chinese Acad Sci, Beijing, Peoples R China 9.Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou, Fujian, Peoples R China
推荐引用方式 GB/T 7714	Qi, Shi-Lian,Lin, Qing-Fang,Feng, Xuan-Jun,et al. IDD16 negatively regulates stomatal initiation via trans-repression of SPCH in Arabidopsis[J]. PLANT BIOTECHNOLOGY JOURNAL,2019,17(7):1446-1457.
APA	Qi, Shi-Lian.,Lin, Qing-Fang.,Feng, Xuan-Jun.,Han, Hui-Ling.,Liu, Jie.,...&Hua, Xue-Jun.(2019).IDD16 negatively regulates stomatal initiation via trans-repression of SPCH in Arabidopsis.PLANT BIOTECHNOLOGY JOURNAL,17(7),1446-1457.
MLA	Qi, Shi-Lian,et al."IDD16 negatively regulates stomatal initiation via trans-repression of SPCH in Arabidopsis".PLANT BIOTECHNOLOGY JOURNAL 17.7(2019):1446-1457.