A missense mutation of STERILE APETALA leads to female sterility in Chinese cabbage (Brassica campestris ssp. pekinensis)

doi:10.1007/s00497-019-00368-7

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	A missense mutation of STERILE APETALA leads to female sterility in Chinese cabbage (Brassica campestris ssp. pekinensis)
	Liu, Wenjie ; Huang, Shengnan ; Liu, Zhiyong ; Lou, Tengxue 2; Tan, Chong ; Wang, Yiheng ; Feng, Hui
刊名	PLANT REPRODUCTION
	2019
卷号	32 期号:2 页码:217-228
关键词	Chinese cabbage EMS mutagenesis Female-sterile STERILE APETALA
ISSN号	2194-7953
DOI	10.1007/s00497-019-00368-7
文献子类	Article
英文摘要	Flower development is essential for the sexual reproduction and crop yield of plants. Thus, a better understanding of plant sterility from the perspective of morphological and molecular genetics is imperative. In our previous study, a recessive female-sterile Chinese cabbage mutant fsm was obtained from a doubled haploid line FT' via an isolated microspore culture combined with EMS mutagenesis. Pistil aniline blue staining and stigma scanning observation showed that the growth of the stigma papillar cells and pollen tubes of the mutant fsm were normal. Therefore, the female sterility was due to abnormal development of the ovules. To map the mutant fsm, 3108 F-2 individuals were selected for linkage analysis. Two closely linked markers, Indel-I2 and Indel-I7, were localized on the flanking region of fsm at distances of 0.05cM and 0.06cM, respectively. The physical distance between Indel-I2 and Indel-I7 was1376kb, with 107 genes remaining in the target region. This region was located on the chromosome A04 centromere, on which low recombination rates and a high frequency of repetitive sequences were present. Whole-genome re-sequencing detected a single-nucleotide (C-to-A) transition (TCG/TAG) on the exon of BraA04001030, resulting in a premature stop codon. Genotyping revealed that the female-sterile phenotype was fully cosegregated with this SNP. BraA04001030 encodes a homologue of STERILE APETALA(SAP) transcriptional regulator, which plays vital roles in floral development. The results of the present study suggest that BraA04001030 is a strong candidate gene for fsm and provide the basis for exploring the molecular mechanism underlying female sterility in Chinese cabbage.
学科主题	Plant Sciences ; Reproductive Biology
电子版国际标准刊号	2194-7961
出版地	NEW YORK
WOS关键词	REGULATES CELL EXPANSION ; MADS-BOX GENES ; ORGAN SIZE ; OVULE DEVELOPMENT ; ROS HOMEOSTASIS ; ARABIDOPSIS ; AUXIN ; GAMETOPHYTE ; FAMILY ; SEED
WOS研究方向	Plant Sciences ; Reproductive Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000466918500008
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31672144]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/19714]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Shenyang Agr Univ, Dept Hort, 120 Dongling Rd, Shenyang 110866, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Liu, Wenjie,Huang, Shengnan,Liu, Zhiyong,et al. A missense mutation of STERILE APETALA leads to female sterility in Chinese cabbage (Brassica campestris ssp. pekinensis)[J]. PLANT REPRODUCTION,2019,32(2):217-228.
APA	Liu, Wenjie.,Huang, Shengnan.,Liu, Zhiyong.,Lou, Tengxue.,Tan, Chong.,...&Feng, Hui.(2019).A missense mutation of STERILE APETALA leads to female sterility in Chinese cabbage (Brassica campestris ssp. pekinensis).PLANT REPRODUCTION,32(2),217-228.
MLA	Liu, Wenjie,et al."A missense mutation of STERILE APETALA leads to female sterility in Chinese cabbage (Brassica campestris ssp. pekinensis)".PLANT REPRODUCTION 32.2(2019):217-228.