An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA Stability and Translation in Arabidopsis and Maize

doi:10.1105/tpc.18.00946

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	An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA Stability and Translation in Arabidopsis and Maize
	Jiang, Jingjing 2; Chai, Xin 2; Manavski, Nikolay 1,6; Williams-Carrier, Rosalind ; He, Baoye ; Brachmann, Aandreas 4; Ji, Daili ; Ouyang, Min ; Liu, Yini ; Barkan, Alice 3
刊名	PLANT CELL
	2019
卷号	31 期号:6 页码:1308-1327
ISSN号	1040-4651
DOI	10.1105/tpc.18.00946
文献子类	Article
英文摘要	A key characteristic of chloroplast gene expression is the predominance of posttranscriptional control via numerous nucleus-encoded RNA binding factors. Here, we explored the essential roles of the S1-domain-containing protein photosynthetic electron transfer B (petB)/ petD Stabilizing Factor (BSF) in the stabilization and translation of chloroplast mRNAs. BSF binds to the intergenic region of petB-petD, thereby stabilizing 39 processed petB transcripts and stimulating petD translation. BSF also binds to the 59 untranslated region of petA and activates its translation. BSF displayed nucleic-acid-melting activity in vitro, and its absence induces structural changes to target RNAs in vivo, suggesting that BSF functions as an RNA chaperone to remodel RNA structure. BSF physically interacts with the pentatricopeptide repeat protein Chloroplast RNA Processing 1 (AtCRP1) and the ribosomal release factor-like protein Peptide chain Release Factor 3 (PrfB3), whose established RNA ligands overlap with those of BSF. In addition, PrfB3 stimulated the RNA binding ability of BSF in vitro. We propose that BSF and PrfB3 cooperatively reduce the formation of secondary RNA structures within target mRNAs and facilitate AtCRP1 binding. The translation activation function of BSF for petD is conserved in Arabidopsis (Arabidopsis thaliana) and maize (Zea mays), but that for petA operates specifically in Arabidopsis. Our study sheds light on the mechanisms by which RNA binding proteins cooperatively regulate mRNA stability and translation in chloroplasts.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
电子版国际标准刊号	1532-298X
出版地	CARY
WOS关键词	PENTATRICOPEPTIDE REPEAT PROTEIN ; ESCHERICHIA-COLI CSPE ; ACID MELTING ACTIVITY ; BINDING DOMAIN ; PHOTOSYSTEM-II ; TRANSCRIPTION ANTITERMINATION ; RIBOSOMAL-SUBUNITS ; SPLICING FACTOR ; NUCLEAR GENES ; COMPLEX
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
语种	英语
出版者	OXFORD UNIV PRESS INC
WOS记录号	WOS:000471166900025
资助机构	Chinese Academy of Sciences (CAS) Strategic Priority Research Program [XDB17030100] ; Ministry of Science and Technology of the People's Republic of China (Chinese Ministry of Science and Technology) Major State Basic Research Development Program [2015CB150101] ; German Science Foundation (DFG)German Research Foundation (DFG) [TRR175 A03] ; US National Science FoundationNational Science Foundation (NSF) [IOS-1339130]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/19668]
专题	中科院光生物学重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Photosynth Res Ctr, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China 3.Ludwig Maximilians Univ Munchen, Biozentrum, Plant Mol Biol, D-82152 Planegg Martinsried, Germany 4.Univ Oregon, Inst Mol Biol, Eugene, OR 97403 USA 5.Ludwig Maximilians Univ Munchen, Genet, Fac Biol, D-82152 Planegg Martinsried, Germany 6.Henan Univ, Key Lab Plant Stress Biol, State Key Lab Cotton Biol, Sch Life Sci, Kaifeng 475004, Peoples R China 7.CNRS, Inst Biol Mol 5 Plantes, 12 Rue Gen Zimmer, F-67084 Strasbourg, France
推荐引用方式 GB/T 7714	Jiang, Jingjing,Chai, Xin,Manavski, Nikolay,et al. An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA Stability and Translation in Arabidopsis and Maize[J]. PLANT CELL,2019,31(6):1308-1327.
APA	Jiang, Jingjing.,Chai, Xin.,Manavski, Nikolay.,Williams-Carrier, Rosalind.,He, Baoye.,...&Chi, Wei.(2019).An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA Stability and Translation in Arabidopsis and Maize.PLANT CELL,31(6),1308-1327.
MLA	Jiang, Jingjing,et al."An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA Stability and Translation in Arabidopsis and Maize".PLANT CELL 31.6(2019):1308-1327.