Anti-Inflammatory and Antioxidant Effects of Carpesium cernuum L. Methanolic Extract in LPS-Stimulated RAW 264.7 Macrophages

doi:10.1155/2020/3164239

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	Anti-Inflammatory and Antioxidant Effects of Carpesium cernuum L. Methanolic Extract in LPS-Stimulated RAW 264.7 Macrophages
	Park, Yea-Jin ; Cheon, Se-Yun ; Lee, Dong-Sung ; Cominguez, Divina C.; Zhang, Zhiyun 4; Lee, Sangwoo 5; An, Hyo-Jin
刊名	MEDIATORS OF INFLAMMATION
	2020
卷号	2020
ISSN号	0962-9351
DOI	10.1155/2020/3164239
文献子类	Article
英文摘要	A hypernomic reaction or an abnormal inflammatory process could cause a series of diseases, such as cardiovascular disease, neurodegeneration, and cancer. Additionally, oxidative stress has been identified to induce severe tissue injury and inflammation.Carpesium cernuumL. (C.cernuum) is a Chinese folk medicine used for its anti-inflammatory, analgesic, and detoxifying properties. However, the underlying molecular mechanism of C.cernuumin inflammatory and oxidative stress conditions remains largely unknown. The aim of this study was to examine the effects of a methanolic extract of C.cernuum(CLME) on lipopolysaccharide- (LPS-) induced RAW 264.7 mouse macrophages and a sepsis mouse model. The data presented in this study indicated that CLME inhibited LPS-induced production of proinflammatory mediators such as nitric oxide (NO) and prostaglandin E-2(PGE(2)) in RAW 264.7 cells. CLME treatment also reduced reactive oxygen species (ROS) generation and enhanced the expression of heme oxygenase-1 (HO-1) protein in a dose-dependent manner in the LPS-stimulated RAW 264.7 cells. Moreover, CLME treatment abolished the nuclear translocation of nuclear factor-kappa B (NF-kappa B), enhanced the activation of nuclear factor-erythroid 2 p45-related factor 2 (Nrf2), and reduced the expression of extracellular signal-related kinase (ERK) and ERK kinase (MEK) phosphorylation in LPS-stimulated RAW 264.7 cells. These outcomes implied that CLME could be a potential antioxidant and anti-inflammatory agent.
学科主题	Cell Biology ; Immunology
电子版国际标准刊号	1466-1861
出版地	LONDON
WOS关键词	NF-KAPPA-B ; INDUCED HEPATOCELLULAR-CARCINOMA ; INDUCED INFLAMMATORY RESPONSES ; OXIDATIVE STRESS ; RAW264.7 CELLS ; ACTIVATION ; INHIBITION ; MAPK ; INACTIVATION ; JAK/STAT
WOS研究方向	Cell Biology ; Immunology
语种	英语
出版者	HINDAWI LTD
WOS记录号	WOS:000561633800001
资助机构	Basic Science Program through the National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning [NRF-2019R1A2C4070234] ; KRIBB Initiative Program of the Republic of Korea
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21620]
专题	系统与进化植物学国家重点实验室
作者单位	1.Sangji Univ, Dept Pharmacol, Coll Korean Med, Wonju 26339, Gangwon Do, South Korea 2.Pusan Natl Univ, Sch Korean Med, Dept Korean Med Sci, Yangsan 50612, Gyeongnam, South Korea 3.Pusan Natl Univ, Hlth Aging Korean Med Res Ctr, Yangsan 50612, Gyeongnam, South Korea 4.Chosun Univ, Coll Pharm, 309 Pilmun Daero, Gwangju 61452, South Korea 5.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 6.Korea Res Inst Biosci & Biotechnol, Int Biol Mat Res Ctr, Daejeon 34141, South Korea
推荐引用方式 GB/T 7714	Park, Yea-Jin,Cheon, Se-Yun,Lee, Dong-Sung,et al. Anti-Inflammatory and Antioxidant Effects of Carpesium cernuum L. Methanolic Extract in LPS-Stimulated RAW 264.7 Macrophages[J]. MEDIATORS OF INFLAMMATION,2020,2020.
APA	Park, Yea-Jin.,Cheon, Se-Yun.,Lee, Dong-Sung.,Cominguez, Divina C..,Zhang, Zhiyun.,...&An, Hyo-Jin.(2020).Anti-Inflammatory and Antioxidant Effects of Carpesium cernuum L. Methanolic Extract in LPS-Stimulated RAW 264.7 Macrophages.MEDIATORS OF INFLAMMATION,2020.
MLA	Park, Yea-Jin,et al."Anti-Inflammatory and Antioxidant Effects of Carpesium cernuum L. Methanolic Extract in LPS-Stimulated RAW 264.7 Macrophages".MEDIATORS OF INFLAMMATION 2020(2020).