Terpyridine Zn(II) Complexes with Azide Units for Visualization of Histone Deacetylation in Living Cells under STED Nanoscopy

doi:10.1021/acssensors.1c01287

	Terpyridine Zn(II) Complexes with Azide Units for Visualization of Histone Deacetylation in Living Cells under STED Nanoscopy
	Du, Wei 3,4,5; Pan, Dayi 3; Xiang, Pan 3; Xiong, Chaoya 1; Zhang, Mingzhu 1; Zhang, Qiong 1; Tian, Yupeng 1; Zhang, Zhongping 1,6; Chen, Bo 2; Luo, Kui 3,7
刊名	ACS SENSORS
	2021-11-26
卷号	6
关键词	Zn complexes azide units histone probe STED nanoscopy super-resolution imaging
ISSN号	2379-3694
DOI	10.1021/acssensors.1c01287
通讯作者	Chen, Bo(bo.chen@tongji.cdu.cn) ; Tian, Xiaohe(xiaohe.t@wchscu.cn)
英文摘要	Histones are the alkali proteins in eukaryotic somatic chromatin cells which constitute the nucleosome structure together with DNA. Their abnormality is often associated with multiple tumorigenesis and other human diseases. Nevertheless, a simple and efficient super-resolution method to visualize histone distribution at the subcellular level is still unavailable. Herein, a Zn(II) terpyridine complex with rich-electronic azide units, namely, TpZnA-His, was designed and synthesized. The initial in vitro and in silico studies suggested that this complex is able to detect histones rapidly and selectively via charge-charge interactions with the histone H3 subunit. Its live cell nuclear localization, red-emission tail, and large Stokes shift allowed super-resolution evaluation of histone distributions with a clear distinction against nuclear DNA. We were able to quantitatively conclude three histone morphology alternations in live cells including condensation, aggregation, and cavity during activating histone acetylation. This work offers a better understanding as well as a versatile tool to study histone-involved gene transcription, signal transduction, and differentiation in cells.
资助项目	National Natural Science Foundation of China[81621003] ; National Natural Science Foundation of China[321710012221871003] ; Postdoctoral Research Funding Schemes of Jiangsu Province (2021)
WOS关键词	LABEL-FREE ; FLUORESCENCE
WOS研究方向	Chemistry ; Science & Technology - Other Topics
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000755689100012
资助机构	National Natural Science Foundation of China ; Postdoctoral Research Funding Schemes of Jiangsu Province (2021)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/127503]
专题	中国科学院合肥物质科学研究院
通讯作者	Chen, Bo; Tian, Xiaohe
作者单位	1.Anhui Univ, Sch Life Sci, Inst Phys Sci & Informat Technol, Dept Chem, Hefei 230000, Peoples R China 2.Tongji Univ, Sch Mat Sci & Engn, Shanghai 201804, Peoples R China 3.Sichuan Univ, West China Hosp, Huaxi Magnet Resonance Res Ctr HMRRC, Dept Radiol,Funct & Mol Imaging Key Lab Sichuan P, Chengdu 610041, Peoples R China 4.Nanjing Tech Univ NanjingTech, Key Lab Flexible Elect KLOFE, Nanjing 211816, Peoples R China 5.Nanjing Tech Univ NanjingTech, Inst Adv Mat IAM, Nanjing 211816, Peoples R China 6.Chinese Acad Sci, CAS Ctr Excellence Nanosci, Inst Intelligent Machines, Hefei 230031, Peoples R China 7.Chinese Acad Med Sci, Res Unit Psychoradiol, Chengdu 610041, Peoples R China
推荐引用方式 GB/T 7714	Du, Wei,Pan, Dayi,Xiang, Pan,et al. Terpyridine Zn(II) Complexes with Azide Units for Visualization of Histone Deacetylation in Living Cells under STED Nanoscopy[J]. ACS SENSORS,2021,6.
APA	Du, Wei.,Pan, Dayi.,Xiang, Pan.,Xiong, Chaoya.,Zhang, Mingzhu.,...&Tian, Xiaohe.(2021).Terpyridine Zn(II) Complexes with Azide Units for Visualization of Histone Deacetylation in Living Cells under STED Nanoscopy.ACS SENSORS,6.
MLA	Du, Wei,et al."Terpyridine Zn(II) Complexes with Azide Units for Visualization of Histone Deacetylation in Living Cells under STED Nanoscopy".ACS SENSORS 6(2021).