Analysis of Gut Microbiota Composition in Lung Adenocarcinoma Patients with TCM Qi-Yin Deficiency

doi:10.1142/S0192415X21500786

	Analysis of Gut Microbiota Composition in Lung Adenocarcinoma Patients with TCM Qi-Yin Deficiency
	Chen, Jiabin 3; Wang, Sheng 2; Shen, Jianfei 4; Hu, Qinqin 3; Zhang, Yongjun 1; Ma, Dehua 4; Chai, Kequn 3
刊名	AMERICAN JOURNAL OF CHINESE MEDICINE
	2021
卷号	49
关键词	Lung Adenocarcinoma Traditional Chinese Medicine Qi-Yin Deficiency Gut Microbiota Diagnostic Model
ISSN号	0192-415X
DOI	10.1142/S0192415X21500786
通讯作者	Ma, Dehua(madh@enzemed.com) ; Chai, Kequn(ckq_official@163.com)
英文摘要	In Lung adenocarcinoma (ADC), Qi-Yin deficiency syndrome (QY) is the most common Traditional Chinese medicine (TCM) syndrome. This study aimed to investigate the diversity and composition of gut microbiota in ADC patients with QY syndrome. 90 stool samples, including 30 healthy individuals (H), 30 ADC patients with QY syndrome, and 30 ADC patients with another syndrome (O) were collected. Then, 16s-RNA sequencing was used to analyze stool samples to clarify the structure of gut microbiota, and linear discriminant analysis (LDA) effect size (LEfSe) was applied to identify biomarkers for ADC with QY syndrome. Logistic regression analysis was performed to establish a diagnostic model for the diagnosis of QY syndrome in ADC patients, which was assessed with the AUC. Finally, 20 fecal samples (QY: 10; O: 10) were analyzed with Metagenomics to validate the diagnostic model. The alpha diversity and beta diversity demonstrated that the structure of gut microbiota in the QY group was different from that of the H group and O group. In the QY group, the top 3 taxonomies at phylum level were Firmicutes, Bacteroidetes, and Proteobacteria, and at genus level were Faecalibacterium, Prevotella_9, and Bifidobacterium. LEfSe identified Prevotella_9 and Streptococcus might be the biomarkers for QY syndrome. A diagnostic model was constructed using those 2 genera with the AUC = 0.801, similar to the AUC based on Metagenomics (0.842). The structure of gut microbiota in ADC patients with QY syndrome was investigated, and a diagnostic model was developed for the diagnosis of QY syndrome in ADC patients, which provides a novel idea for the understanding and diagnosis of TCM syndrome.
资助项目	National Natural Science Foundation of China[81673781] ; Key R&D plan of Zhejiang Province[2015C03033] ; Zhejiang provincial fund for outstanding young talents of traditional Chinese Medicine[2019ZQ006]
WOS关键词	TRADITIONAL CHINESE MEDICINE ; ADJUNCTIVE THERAPY ; CANCER ; PREVOTELLA ; SURVIVAL ; INSIGHTS ; BIOLOGY
WOS研究方向	Integrative & Complementary Medicine ; General & Internal Medicine
语种	英语
出版者	WORLD SCIENTIFIC PUBL CO PTE LTD
WOS记录号	WOS:000720336900005
资助机构	National Natural Science Foundation of China ; Key R&D plan of Zhejiang Province ; Zhejiang provincial fund for outstanding young talents of traditional Chinese Medicine
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/126620]
专题	中国科学院合肥物质科学研究院
通讯作者	Ma, Dehua; Chai, Kequn
作者单位	1.Univ Chinese Acad Sci, Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 2.Jinhua Guangfu Hosp, Dept Resp, Jinhua 321000, Zhejiang, Peoples R China 3.Tongde Hosp Zhejiang, Dept Oncol, 234 Gu Cui Rd, Hangzhou 310012, Zhejiang, Peoples R China 4.Taizhou Hosp, Dept Thorac Surg, 150 Ximen St, Taizhou 310012, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Chen, Jiabin,Wang, Sheng,Shen, Jianfei,et al. Analysis of Gut Microbiota Composition in Lung Adenocarcinoma Patients with TCM Qi-Yin Deficiency[J]. AMERICAN JOURNAL OF CHINESE MEDICINE,2021,49.
APA	Chen, Jiabin.,Wang, Sheng.,Shen, Jianfei.,Hu, Qinqin.,Zhang, Yongjun.,...&Chai, Kequn.(2021).Analysis of Gut Microbiota Composition in Lung Adenocarcinoma Patients with TCM Qi-Yin Deficiency.AMERICAN JOURNAL OF CHINESE MEDICINE,49.
MLA	Chen, Jiabin,et al."Analysis of Gut Microbiota Composition in Lung Adenocarcinoma Patients with TCM Qi-Yin Deficiency".AMERICAN JOURNAL OF CHINESE MEDICINE 49(2021).