A self-quenching fluorescence probe-mediated exponential isothermal amplification system for highly sensitive and specific detection of microRNAs

doi:10.1039/d1cc05522d

	A self-quenching fluorescence probe-mediated exponential isothermal amplification system for highly sensitive and specific detection of microRNAs
	Zhao, Jun 2; Wu, Jiandong 4; Wu, Xiaosong 2,3; Deng, Guoqing 2; Liu, Yong 2; Lin, Francis 1; Zhu, Ling 2
刊名	CHEMICAL COMMUNICATIONS
	2021-11-25
卷号	57
ISSN号	1359-7345
DOI	10.1039/d1cc05522d
通讯作者	Lin, Francis(Francis.Lin@umanitoba.ca) ; Zhu, Ling(zhul@aiofm.ac.cn)
英文摘要	We designed an efficient self-quenching fluorescence probe and constructed this probe-mediated exponential isothermal amplification system for miRNA detection. Owing to the significant improvement in the detective signal-to-background ratio, a wide dynamic range of 9 orders of magnitude and a limit of detection as low as 0.08 aM can be easily achieved in a single step. Furthermore, benefiting from the additional advantages of high specificity and biocompatibility, the proposed method has been demonstrated to be capable of accurately quantifying miRNA biomarkers in serum, which will provide promising perspectives for clinical diagnosis.
WOS关键词	ROLLING CIRCLE AMPLIFICATION ; UNIVERSAL REPORTER ; SERUM
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000719433900001
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/126600]
专题	中国科学院合肥物质科学研究院
通讯作者	Lin, Francis; Zhu, Ling
作者单位	1.Univ Manitoba, Winnipeg, MB R3T 2N2, Canada 2.Chinese Acad Sci, Ctr Engn Technol Res Biomed Opt Instrument, Hefei Inst Phys Sci, Hefei 230031, Peoples R China 3.Univ Sci & Technol China, Hefei 230026, Peoples R China 4.Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Hlth Engn, Shenzhen 518055, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Jun,Wu, Jiandong,Wu, Xiaosong,et al. A self-quenching fluorescence probe-mediated exponential isothermal amplification system for highly sensitive and specific detection of microRNAs[J]. CHEMICAL COMMUNICATIONS,2021,57.
APA	Zhao, Jun.,Wu, Jiandong.,Wu, Xiaosong.,Deng, Guoqing.,Liu, Yong.,...&Zhu, Ling.(2021).A self-quenching fluorescence probe-mediated exponential isothermal amplification system for highly sensitive and specific detection of microRNAs.CHEMICAL COMMUNICATIONS,57.
MLA	Zhao, Jun,et al."A self-quenching fluorescence probe-mediated exponential isothermal amplification system for highly sensitive and specific detection of microRNAs".CHEMICAL COMMUNICATIONS 57(2021).