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	Chemical modification and characterization of gigaporous polystyrene microspheres as rapid separation of proteins base supports
	Qu, Jian-Bo1,2; Zhou, Wei-Qing1; Wei, Wei1,2; Su, Zhi-Guo1; Ma, Guang-Hui1
刊名	JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY
	2008-09-01
卷号	46期号:17页码:5794-5804
关键词	adsorption coatings gigaporous modification polystyrene microspheres protein adsorption
ISSN号	0887-624X
其他题名	J. Polym. Sci. Pol. Chem.
中文摘要	To overcome the disadvantages of protein denaturation and nonspecific adsorption on poly(styrene-divinylbenzene) (PS) matrix as chromatographic supports, gigaporous PS microspheres, which we prepared in a previous study, were chemically modified with poly(vinyl alcohol) (PVA) through two-step reaction. The microspheres were chloroacetylated through Friedel-Crafts acetylation with chloroacetyl chloride and modified with hydrophilic PVA through Williamson reaction afterward. The modified microspheres were characterized by Fourier transform infrared (FTIR) spectra, X-ray photoelectron spectroscopy (XPS), scanning electron microscope (SEM), mercury porosimetry measurements (MPM), BET nitrogen adsorption measurements, laser scanning confocal microscope (LSCM), and protein adsorption experiments. Results showed that PS microspheres were successfully coated with PVA, while the gigaporous structure could be maintained. Consequently, the hydrophilicity and biocompatibility of modified microspheres was greatly improved and nonspecific adsorption of proteins was significantly decreased. The coatings contained only stable chemical bonds (e.g., C-C, C-O-C) and easily derived hydroxyl moieties. The large pores of gigaporous PS microspheres also facilitated the modification by PVA. After further derivation, the coated gigaporous base supports could apply in various modes of chromatography and have great potentials in high-speed protein chromatography. (c) 2008 Wiley Periodicals, Inc.
英文摘要	To overcome the disadvantages of protein denaturation and nonspecific adsorption on poly(styrene-divinylbenzene) (PS) matrix as chromatographic supports, gigaporous PS microspheres, which we prepared in a previous study, were chemically modified with poly(vinyl alcohol) (PVA) through two-step reaction. The microspheres were chloroacetylated through Friedel-Crafts acetylation with chloroacetyl chloride and modified with hydrophilic PVA through Williamson reaction afterward. The modified microspheres were characterized by Fourier transform infrared (FTIR) spectra, X-ray photoelectron spectroscopy (XPS), scanning electron microscope (SEM), mercury porosimetry measurements (MPM), BET nitrogen adsorption measurements, laser scanning confocal microscope (LSCM), and protein adsorption experiments. Results showed that PS microspheres were successfully coated with PVA, while the gigaporous structure could be maintained. Consequently, the hydrophilicity and biocompatibility of modified microspheres was greatly improved and nonspecific adsorption of proteins was significantly decreased. The coatings contained only stable chemical bonds (e.g., C-C, C-O-C) and easily derived hydroxyl moieties. The large pores of gigaporous PS microspheres also facilitated the modification by PVA. After further derivation, the coated gigaporous base supports could apply in various modes of chromatography and have great potentials in high-speed protein chromatography. (c) 2008 Wiley Periodicals, Inc.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Polymer Science
研究领域[WOS]	Polymer Science
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; EXCHANGE PACKING MATERIAL ; PERFUSION CHROMATOGRAPHY ; ADSORPTION ; PARTICLES ; SURFACES ; PHASE ; DEXTRAN ; PEG
收录类别	SCI
原文出处	://WOS:000258981000009
语种	英语
WOS记录号	WOS:000258981000009
公开日期	2013-10-08
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/2835]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Qu, Jian-Bo,Zhou, Wei-Qing,Wei, Wei,et al. Chemical modification and characterization of gigaporous polystyrene microspheres as rapid separation of proteins base supports[J]. JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY,2008,46(17):5794-5804.
APA	Qu, Jian-Bo,Zhou, Wei-Qing,Wei, Wei,Su, Zhi-Guo,&Ma, Guang-Hui.(2008).Chemical modification and characterization of gigaporous polystyrene microspheres as rapid separation of proteins base supports.JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY,46(17),5794-5804.
MLA	Qu, Jian-Bo,et al."Chemical modification and characterization of gigaporous polystyrene microspheres as rapid separation of proteins base supports".JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY 46.17(2008):5794-5804.

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