Preparative isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay

	Preparative isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay
	Liu, Chuang; Lei, Yuqing; Dang, Jun; Wang, Weidong; Zhang, Jing; Mei, Lijuan; Liu, Zenggen; Tao, Yanduo; Shao, Yun
刊名	JOURNAL OF SEPARATION SCIENCE
	2021
卷号	44 期号:7
关键词	free radical inhibitors liquid chromatography medium-pressure chromatography Ribes himalense
英文摘要	The lack of suitable chromatographic purification methods makes it a challenge to effectively isolate the chemical components of traditional Tibetan medicines. Ribes himalense is a rarely studied Tibetan medicine, reputed to have free radical-scavenging effects. In the present work, we utilized it as a model herb to highlight an approach for the separation of 1,1-diphenyl-2-picrylhydrazyl inhibitors via medium-pressure chromatography and two-dimensional reversed-phase/reversed-phase interaction liquid chromatography under the guidance of an online high-performance liquid chromatography-1,1-diphenyl-2-picrylhydrazyl assay. Finally, we obtained two free radical inhibitors (>95% purity) from the R. himalense extract. This is the first report of the rapid isolation of these free radical inhibitors from R. himalense. This method can be useful in quality standard assessment and further pharmacological activity research, and may be used as a reference for the composition research of various natural products.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/60855]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Liu, Chuang,Lei, Yuqing,Dang, Jun,et al. Preparative isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay[J]. JOURNAL OF SEPARATION SCIENCE,2021,44(7).
APA	Liu, Chuang.,Lei, Yuqing.,Dang, Jun.,Wang, Weidong.,Zhang, Jing.,...&Shao, Yun.(2021).Preparative isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay.JOURNAL OF SEPARATION SCIENCE,44(7).
MLA	Liu, Chuang,et al."Preparative isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay".JOURNAL OF SEPARATION SCIENCE 44.7(2021).