A ratiometric fluorescence assay for bleomycin based on Cu2+-triggered cascade reactions and nanoparticle-mediated autocatalytic reactions

	A ratiometric fluorescence assay for bleomycin based on Cu2+-triggered cascade reactions and nanoparticle-mediated autocatalytic reactions
	Guo, Fujin; Xia, Meng; Sun, Jing; Zhu, Shuyun
刊名	NEW JOURNAL OF CHEMISTRY
	2021
卷号	45 期号:30
英文摘要	A simple strategy has been designed for ratiometric fluorescence sensing of BLM on the basis of Cu2+-triggered fluorogenic reactions coupled with nanoparticle-mediated autocatalytic reactions. On one hand, Cu2+ can oxidize ascorbic acid (AA) to produce dehydroascorbic acid (DHAA), which condensates with o-phenylenediamine (OPD) to form fluorescent 3-(dihydroxyethyl) furo[3,4-b]quinoxaline-1-one (DFQ) with strong emission at 436 nm. On the other hand, Cu2+ and OPD undergo a redox reaction to form fluorescent 2,3-diaminophenazine (DAP) showing strong emission at 565 nm and copper nanoparticles (CuNPs) that further catalyze this redox reaction. A ratiometric fluorescence system comes into being due to these two well-resolved emission peaks. Ascribed to the complexation of BLM with Cu2+, larger CuNPs are obtained which weaken the autocatalytic reaction between Cu2+ and OPD. Thus, the fluorescence intensities at 565 nm belonging to DAP decrease while those at 436 nm corresponding to DFQ change slightly. With the fluorescence intensity ratio (F-436/F-565) as a readout, BLM can be detected selectively and sensitively with a detection limit of 50 pM.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/60784]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Guo, Fujin,Xia, Meng,Sun, Jing,et al. A ratiometric fluorescence assay for bleomycin based on Cu2+-triggered cascade reactions and nanoparticle-mediated autocatalytic reactions[J]. NEW JOURNAL OF CHEMISTRY,2021,45(30).
APA	Guo, Fujin,Xia, Meng,Sun, Jing,&Zhu, Shuyun.(2021).A ratiometric fluorescence assay for bleomycin based on Cu2+-triggered cascade reactions and nanoparticle-mediated autocatalytic reactions.NEW JOURNAL OF CHEMISTRY,45(30).
MLA	Guo, Fujin,et al."A ratiometric fluorescence assay for bleomycin based on Cu2+-triggered cascade reactions and nanoparticle-mediated autocatalytic reactions".NEW JOURNAL OF CHEMISTRY 45.30(2021).