OsciDrop: A Versatile Deterministic Droplet Generator

doi:10.1021/acs.analchem.1c04852

CORC > 力学研究所 > 中国科学院力学研究所 > 非线性力学国家重点实验室

	OsciDrop: A Versatile Deterministic Droplet Generator
	Ye, Shun 8,9; Li, Caiming 7,9; Zheng, Xu 6; Huang, Weihang 5; Tao, Yi 9; Yu, Yanghuan 9; Yang, Limin 4; Lan, Ying 9; Ma, Liang 3; Bian, Shengtai 2
刊名	ANALYTICAL CHEMISTRY
	2022-01-21
页码	8
ISSN号	0003-2700
DOI	10.1021/acs.analchem.1c04852
通讯作者	Zheng, Xu(zhengxu@lnm.imech.ac.cn) ; Ma, Liang(liangma.chem@gmail.com) ; Bian, Shengtai(stbian@bsu.edu.cn) ; Du, Wenbin(wenbin@im.ac.cn)
英文摘要	This paper describes OsciDrop, a versatile chip-free droplet generator used to produce size-tunable droplets on demand. Droplet generation is fundamental to miniaturized analysis. We designed OsciDrop to segment the fluid flowing out of the orifice of a disposable pipette tip into droplets by oscillating its distal end underneath an immiscible continuous phase. We described the theoretical model and investigated the effect of flow rate, oscillating amplitude, frequency, and waveform on droplet generation. Our study revealed a previously underexplored Weber number-dominated regime that leverages inertial force instead of viscous force to generate droplets. The same pipette tip allowed robust and deterministic generation of monodisperse droplets with programmable sizes ranging from 200 pL to 2 mu L by asymmetrical oscillation. We validated this platform with two droplet-based nucleic acid amplification tests: a digital loop-mediated isothermal amplification assay for absolute quantification of African swine fever virus and a multi-volume digital polymerase chain reaction assay for the high dynamic range measurement of human genomic DNA. The OsciDrop method opens a facile avenue to miniaturization, integration, and automation, exhibiting full accessibility for digital molecular diagnostics.
资助项目	National Key Research and Development Program of China[2021YFC2301000] ; National Key Research and Development Program of China[2021YFA0717000] ; National Key Research and Development Program of China[2018YFC0310703] ; National Key Research and Development Program of China[2016YFE0205800] ; National Natural Science Foundation of China[21822408] ; National Natural Science Foundation of China[91951103] ; National Natural Science Foundation of China[12072350] ; National Natural Science Foundation of China[11832017] ; CAS Strategic Priority Research Program[XDB22040403]
WOS关键词	MICROFLUIDICS ; BIOLOGY
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000746929200001
资助机构	National Key Research and Development Program of China ; National Natural Science Foundation of China ; CAS Strategic Priority Research Program
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/88402]
专题	力学研究所_非线性力学国家重点实验室
通讯作者	Zheng, Xu; Ma, Liang; Bian, Shengtai; Du, Wenbin
作者单位	1.Univ Chinese Acad Sci, Savaid Med Sch, Beijing 100049, Peoples R China 2.Beijing Sport Univ, Sch Sport Sci, Beijing 100084, Peoples R China 3.Dawei Biotechnol Co Ltd, Beijing 100085, Peoples R China 4.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 5.Chinese Univ Hong Kong, Dept Linguist & Modern Languages, Hong Kong 999077, Peoples R China 6.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China 7.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 8.Penn State Univ, Coll Engn, Biomed Engn Dept, University Pk, PA 16802 USA 9.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Ye, Shun,Li, Caiming,Zheng, Xu,et al. OsciDrop: A Versatile Deterministic Droplet Generator[J]. ANALYTICAL CHEMISTRY,2022:8.
APA	Ye, Shun.,Li, Caiming.,Zheng, Xu.,Huang, Weihang.,Tao, Yi.,...&Du, Wenbin.(2022).OsciDrop: A Versatile Deterministic Droplet Generator.ANALYTICAL CHEMISTRY,8.
MLA	Ye, Shun,et al."OsciDrop: A Versatile Deterministic Droplet Generator".ANALYTICAL CHEMISTRY (2022):8.