Genomic Characterization of a Novel Tenericutes Bacterium from Deep-Sea Holothurian Intestine

doi:10.3390/microorganisms8121874

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 深海生物蛋白质组学及分子生物学研究组

	Genomic Characterization of a Novel Tenericutes Bacterium from Deep-Sea Holothurian Intestine
	Zhu, Fang-Chao 1,2; Lian, Chun-Ang 1,2; He, Li-Sheng 1
刊名	MICROORGANISMS
	2020-12-01
卷号	8 期号:12 页码:16
关键词	deep-sea holothurian gut symbiont CRISPR Tenericutes
DOI	10.3390/microorganisms8121874
英文摘要	Intestinal bacterial communities are highly relevant to the digestion, nutrition, growth, reproduction, and immunity of animals, but little is known about the composition and function of intestinal microbiota in deep-sea invertebrates. In this study, the intestinal microbiota of six holothurian Molpadia musculus were investigated, showing that their midguts were predominantly occupied by Izemoplasmatales bacteria. Using metagenomic sequencing, a draft genome of 1,822,181 bp was successfully recovered. After comparison with phylogenetically related bacteria, genes involved in saccharide usage and de novo nucleotide biosynthesis were reduced. However, a set of genes responsible for extracellular nucleoside utilization and 14 of 20 amino acid synthesis pathways were completely retained. Under oligotrophic condition, the gut-associated bacterium may make use of extracellular DNA for carbon and energy supplement, and may provide essential amino acids to the host. The clustered regularly interspaced short palindromic repeat (CRISPR) and restriction-modification (RM) systems presented in the genome may provide protection against invading viruses. A linear azol(in)e-containing peptide gene cluster for bacteriocin synthesize was also identified, which may inhibit the colonization and growth of harmful bacteria. Known virulence factors were not found by database searching. On the basis of its phylogenetic position and metabolic characteristics, we proposed that the bacterium represented a novel genus and a novel family within the Izemoplasmatales order and suggested it be named "Candidatus Bathyoplasma sp. NZ". This was the first time describing host-associated Izemoplasmatales.
资助项目	National Key Research and Development Program of China[2018YFC0309804] ; National Key Research and Development Program of China[2016YFC0302504] ; National Key Research and Development Program of China[2016YFC0304905]
WOS关键词	MULTIPLE SEQUENCE ALIGNMENT ; ORGANIC-MATTER ; PHYLOGENOMIC ANALYSIS ; GUT ; CONTRACTILE ; ANNOTATION ; PREDICTION ; ALGORITHM ; EVOLUTION ; QUALITY
WOS研究方向	Microbiology
语种	英语
出版者	MDPI
WOS记录号	WOS:000602533700001
资助机构	National Key Research and Development Program of China
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/8475]
专题	深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组
通讯作者	He, Li-Sheng
作者单位	1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 101408, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Fang-Chao,Lian, Chun-Ang,He, Li-Sheng. Genomic Characterization of a Novel Tenericutes Bacterium from Deep-Sea Holothurian Intestine[J]. MICROORGANISMS,2020,8(12):16.
APA	Zhu, Fang-Chao,Lian, Chun-Ang,&He, Li-Sheng.(2020).Genomic Characterization of a Novel Tenericutes Bacterium from Deep-Sea Holothurian Intestine.MICROORGANISMS,8(12),16.
MLA	Zhu, Fang-Chao,et al."Genomic Characterization of a Novel Tenericutes Bacterium from Deep-Sea Holothurian Intestine".MICROORGANISMS 8.12(2020):16.